Recombinant Human PSP Protein, CF

Product Discontinued

Recombinant Human PSP Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit LPS-induced TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. 5 μg/mL of rhPSP will inhibit 30-60% of the TNF-alpha secretion induced by 0.5 ng/mL of LPS.
• Source: Mouse myeloma cell line, NS0-derived human PSP protein
  Leu21-Ile249, with a C-terminal 6-His tag
• Accession #: Q96DR5
• N-terminal Sequence: Leu21
• Protein/Peptide Type: Recombinant Proteins
• Gene: BPIFA2
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 25.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PSP Protein, CF

• bA49G10.1
• BPIFA2
• C20orf70
• chromosome 20 open reading frame 70
• Parotid secretory protein
• PSP
• short palate, lung and nasal epithelium carcinoma associated 2
• short palate, lung and nasal epithelium carcinoma-associated protein 2
• SPLUNC2

Background

Parotid secretory protein (PSP; also short palate, lung, nasal epithelium carcinoma-associated protein 2 or SPLUNC2 and C20orf70) is a 36 ‑ 40 kDa secreted glycoprotein and member of the Bactericidal permeability-increasing (BPI)/Lipopolysaccharide-binding protein (LBP)/PLUNC superfamily and PLUNC family of proteins (1). Human PSP is synthesized as a 249 amino acid (aa) precursor that contains an 18 aa signal sequence and a 231 aa mature chain. The mature chain contains one BPI/LBS/PLUNC domain (aa 62 ‑ 220) and two potential sites for N-linked glycosylation. Mature human PSP is 31% and 26% aa identical to mature mouse and rat PSP, respectively. Human PSP is expressed in the parotid and submandibular glands in ductal epithelial cells and acinar cells (1 - 2). PSP has been shown to exhibit bacteristatic and bactericidal effects on Pseudomonas aerginosa (1). In addition, PSP‑derived peptides inhibit the binding of endotoxin to LBP and inhibit the endotoxin‑stimulated secretion of tumor necrosis factor alpha from macrophages (1, 3). These findings suggest that PSP peptides can serve as templates for the design of novel anti-inflammatory peptides (3). One study showed that peptide GL‑13 induced bacterial matting, suggesting passive agglutination of bacteria (4). GL-13 was shown to agglutinate Gram negative bacteria P. aerginosa and Aggregatibacter (Actinobacillus) actinomycetemcomitans, Gram positive Streptococcus gordonii and uncoated sheep erythrocytes (4). The agglutination leads to increased clearance by host phagocytic cells.

1. Geetha, C. et al. (2003) Biochem. Soc. Trans. 31:815.
2. Bingle, C.D. and S.-U. Gorr (2004) Int. J. Biochem. Cell Biol. 36:2144.
3. Geetha, C. et al. (2005) J. Dent. Res. 84:149.
4. Gorr, S.-U. et al. (2008) Peptides 29:2118.

Bioinformatics

• Gene Symbol: BPIFA2
• Uniprot:
  • Q96DR5(Human)