Recombinant Human Podocin/NPHS2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Podocin/NPHS2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When recombinant human Nephrin is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human Podocin/NPHS2 binds with a typical ED₅₀ of 0.8-4 μg/mL.
• Source: E. coli-derived human Podocin/NPHS2 protein
  Lys259-Leu383, with an N-terminal Met and a C-terminal 6-His tag
• Accession #: Q9NP85
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 13 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Podocin/NPHS2 Protein, CF

• Nephrosis 2
• nephrosis 2, idiopathic, steroid-resistant (podocin)
• NPHS2
• PDCN
• PDCNSRN1podocin
• Podocin
• SRN1

Background

Podocin, encoded by the NPHS2 gene, is an approximately 50 kDa membrane protein that plays an important role in podocyte function in the kidney. Loss of Podocin function results in albuminuria, hypercholesterolemia, hypertension, and renal failure (1). Human Podocin consists of a 102 amino acid (aa) cytoplasmic domain, a 21 aa intramembrane segment, and a second 262 aa cytoplasmic domain (2, 3). Alternative splicing generates a short isoform with a 68 aa deletion in the second cytoplasmic domain (4, 5). Within aa 259-383 (the region common to both isoforms), human Podocin shares 90% aa sequence identity with mouse and rat Podocin. Podicin localizes to areas of cell-cell contact between podocytes in the renal glomerulus (2, 6, 7). It associates into oligomers and forms complexes with Nephrin, CAR, ZO-1, and the cation ion channel TRPC6 (6-9). It contributes to podocyte function by regulating the activation of TRPC6 and Nephrin mediated signaling (8, 9). Multiple polymorphisms in NPHS2 are associated with steroid-resistant nephrotic syndrome (2).

1. Mollet, G. et al. (2009) J. Am. Soc. Nephrol. 20:2181.
2. Boute, N. et al. (2000) Nat. Genet. 24:349.
3. Roselli, S. et al. (2002) Am. J. Pathol. 160:131.
4. Volker, L.A. et al. (2013) BMC Nephrol. 14:102.
5. Relle, M. et al. (2011) Mod. Pathol. 24:1101.
6. Shono, A. et al. (2007) J. Am. Soc. Nephrol. 18:2525.
7. Schwarz, K. et al. (2001) J. Clin. Invest. 108:1621.
8. Huber, T.B. et al. (2001) J. Biol. Chem. 276:41543.
9. Anderson, M. et al. (2013) Am. J. Physiol. Cell Physiol. 305:C276.

Bioinformatics

• Uniprot:
  • Q9NP85()