Recombinant Human PDGF-AB Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PDGF-AB Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 15-75 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002).
• Source: E. coli-derived human PDGF-AB protein
  

  Human PDGF-A (Ser87 - Thr211) Accession # P04085

  Human PDGF-B (Ser82 - Thr190) Accession # P01127
  N-terminus	C-terminus

• Accession #: P04085.1 (A chain) & P01127.1 (B chain)
• N-terminal Sequence: Ser87 (A chain) & Ser82 (B chain)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14.3 kDa (A chain) & 12.3 kDa (B chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17.5 kDa (A chain) & 12.5 kDa (B chain), reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-AB Protein, CF

• PDGFAB
• PDGF-AB

Background

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta . Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Publications for PDGF-AB (222-AB)(12)

Publications using 222-AB

• Marimoutou, M;Patel, V;Kim, JH;Schaible, N;Alvarez, J;Hughes, J;Obermok, M;Rodríguez, CI;Kallarakal, T;Suki, B;Amin, K;Krishnan, R;Behrsing, HP; The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation Toxics 2024-08-30 [PMID: 39330565] (Bioassay, Human)
• Madsen, SF;Sand, JMB;Juhl, P;Karsdal, M;Thudium, CS;Siebuhr, AS;Bay-Jensen, AC; Fibroblasts are not just fibroblasts: clear differences between dermal and pulmonary fibroblasts' response to fibrotic growth factors Scientific reports 2023-06-09 [PMID: 37296166] (Bioassay, Human)
• MR Hadjichara, BT Roux, E Csomor, CA Feghali-Bo, LA Murray, DL Clarke, MA Lindsay Long intergenic non-coding RNAs regulate human lung fibroblast function: Implications for idiopathic pulmonary fibrosis Sci Rep, 2019-04-15;9(1):6020. 2019-04-15 [PMID: 30988425] (Bioassay, Human)
• SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017-11-27;7(1):16439. 2017-11-27 [PMID: 29180757] (Surface Plasmon Resonance, Human)
• H He, AE Kuriyan, CW Su, M Mahabole, Y Zhang, YT Zhu, HW Flynn, JM Parel, SC Tseng Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3 Sci Rep, 2017-03-02;7(0):43736. 2017-03-02 [PMID: 28252047] (Bioassay, Human)
• Hayes B, Riehle K, Shimizu-Albergine M, Bauer R, Hudkins K, Johansson F, Yeh M, Mahoney W, Yeung R, Campbell J Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis. PLoS ONE, 2014-03-25;9(3):e92925. 2014-03-25 [PMID: 24667490] (Bioassay, Human, Mouse)
• Ding W, Knox TR, Tschumper RC Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood, 2010-07-06;116(16):2984-93. 2010-07-06 [PMID: 20606160] (Bioassay, Human)
• Ball SG, Bayley C, Shuttleworth CA, Kielty CM Neuropilin-1 regulates platelet-derived growth factor receptor signalling in mesenchymal stem cells. Biochem. J., 2010-03-15;427(1):29-40. 2010-03-15 [PMID: 20102335] (Bioassay, Human)
• Li R, Maminishkis A, Wang FE, Miller SS PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5722-32. 2007-12-01 [PMID: 18055825] (Bioassay, Human)
• Gupta S, Ramjaun AR, Haiko P, Wang Y, Warne PH, Nicke B, Nye E, Stamp G, Alitalo K, Downward J Binding of ras to phosphoinositide 3-kinase p110alpha is required for ras-driven tumorigenesis in mice. Cell, 2007-06-01;129(5):957-68. 2007-06-01 [PMID: 17540175] (Bioassay, Mouse)
• Rhodes KE, Raivich G, Fawcett JW The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines. Neuroscience, 2006-04-21;140(1):87-100. 2006-04-21 [PMID: 16631314] (In Vivo, Rat)
• Nagineni CN, Kutty V, Detrick B, Hooks JJ Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta. J. Cell. Physiol., 2005-04-01;203(1):35-43. 2005-04-01 [PMID: 15368539] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P04085.1 (A chain) & P01127.1 (B chain)()