Recombinant Human NPEPPS Protein, CF


There are currently no images for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human NPEPPS Protein, CF Summary

Details of Functionality
Measured by its ability to cleave the fluorogenic peptide substrate, Leu-AMC. The specific activity is >2,500 pmol/min/μg, as measured under the described conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Puromycin-sensitive aminopeptidase/NPEPPS protein
Pro46-Val919, with an N-terminal Met and a C-terminal 10-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Enzymes
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
90-100 kDa, reducing conditions
Read Publication using
6410-ZN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
Assay Procedure
  • Assay Buffer: 25 mM HEPES, 1 mM DTT, pH 7.0
  • Recombinant Human Puromycin‑sensitive aminopeptidase/NPEPPS (rhNPEPPS) (Catalog # 6410-ZN)
  • Substrate: Leu-AMC (Bachem, Catalog # I-1240), 10 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhNPEPPS to 0.2 ng/μL in Assay Buffer.
  2. Dilute Substrate to 20 μM in Assay Buffer.
  3. Load 50 μL of 0.2 ng/μL rhNPEPPS into the microplate and start the reaction by adding 50 μL of Substrate. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Substrate.
  4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, for 5 minutes in kinetic mode.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891.

Per Well:
  • rhNPEPPS: 0.010 µg
  • Substrate: 10 µM


Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NPEPPS Protein, CF

  • AAP-S
  • aminopeptidase puromycin sensitive
  • Cytosol alanyl aminopeptidase
  • EC
  • EC
  • metalloproteinase MP100
  • MP100
  • MP100EC 3.4.11
  • puromycin-sensitive aminopeptidase


NPEPPS, also known as Puromycin-sensitive aminopeptidase and cytosol alanyl aminopeptidase, is a zinc metallopeptidase which hydrolyzes N‑terminal amino acids from its substrates. NPEPPS is expressed in most tissues as a cytoplasmic protein, but a membrane-associated form has been identified in the brain (1, 2). The enzyme has broad substrate specificity but prefers basic and hydrophobic residues in the substrate P1 and P'1 sites (3). NPEPPS is known to hydrolyze dynorphins and enkephalins, and may contribute to the degradation of these peptides in the brain (4). NPEPPS plays a major role in the degradation of toxic polyglutaminyl peptides that are released by proteasomes (5). NPEPPS is also known to degrade the tau protein, which accumulates and polymerizes in some neurodegenerative diseases (6).
  1. Dyer, S.H. et al. (1990) J. Neurochem. 54:547.
  2. McLellan, S. et al. (1988) J. Neurochem. 51:1552.
  3. Johnson, G.D. and L.B. Hersh (1990) Arch. Biochem. Biophys. 276:305.
  4. Safavi, A. and L.B. Hersh (1995) J. Neurochem. 65:389.
  5. Bhutani, N. et al. (2007) EMBO J. 26:1385.
  6. Sengupta, S. et al. (2006) Biochemistry 45:15111.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, IHC
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Pm, Ma-Op, Pm
Applications: WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Mu, Rt
Applications: WB
Species: Hu
Applications: WB, ICC/IF
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Pm, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, IP, Neut
Species: Hu, Rt, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-CS
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Species: Hu
Applications: Enzyme Activity

Publications for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.

Filter By Application
All Applications
Filter By Species
All Species

Reviews for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN) (0)

There are no reviews for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Puromycin-sensitive aminopeptidase/NPEPPS Products

Bioinformatics Tool for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN)

Discover related pathways, diseases and genes to Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN)

Discover more about diseases related to Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN).

Pathways for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN)

View related products by pathway.

PTMs for Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN)

Learn more about PTMs related to Puromycin-sensitive aminopeptidase/NPEPPS (6410-ZN).

Blogs on Puromycin-sensitive aminopeptidase/NPEPPS

There are no specific blogs for Puromycin-sensitive aminopeptidase/NPEPPS, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human NPEPPS Protein, CF and receive a gift card or discount.


Gene Symbol NPEPPS