Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF

Recombinant Human NKG2C/CD159c Fc Chimera (Catalog # 138-NK) binds Recombinant Human CD94 (Catalog # 9270-CD) in a functional ELISA.The ED50 for this effect is 0.3-2.4 µg/mL.

2 μg/lane of Recombinant Human NKG2C/CD159c (Catalog # 138-NK) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 53-62 kDa and 110-120 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind Recombinant Human CD94 (Catalog # 9270-CD) in a functional ELISA. The ED₅₀ for this effect is 0.3‑2.4 µg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human NKG2C/CD159c protein
  

  MD	Human IgG1 (Pro100-Lys330)	IEGR	Human NKG2C (Glu98-Leu231) Accession # P26717
  N-terminus			C-terminus

• Accession #: P26717
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 53-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NKG2C/CD159c Fc Chimera Protein, CF

• CD159 antigen-like family member C
• CD159c antigen
• CD159c
• killer cell lectin-like receptor subfamily C, member 2
• KLRC2
• MGC138244
• NK cell receptor C
• NKG2-C type II integral membrane protein
• NKG2C
• NKG2-C
• NKG2CNKG2-C-activating NK receptor

Background

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8⁺ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8⁺ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C⁺ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12).

1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
6. Braud, V.M. et al. (1998) Nature. 391:795.
7. Lanier, L.L. (1998) Immunity 8:693.
8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
10. Bellón T. et al. (1999) J Immunol 162:3996.
11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
12. Hammer Q. et al. (2018) Nat Immunol. 19:453.

Publications for NKG2C/CD159c/KLRC2 (138-NK)(1)

Publication using 138-NK

• Santos, AJM;van Unen, V;Lin, Z;Chirieleison, SM;Ha, N;Batish, A;Chan, JE;Cedano, J;Zhang, ET;Mu, Q;Guh-Siesel, A;Tomaske, M;Colburg, D;Varma, S;Choi, SS;Christophersen, A;Baghdasaryan, A;Yost, KE;Karlsson, K;Ha, A;Li, J;Dai, H;Sellers, ZM;Chang, HY;Dunn, JCY;Zhang, BM;Mellins, ED;Sollid, LM;Fernandez-Becker, NQ;Davis, MM;Kuo, CJ; A human autoimmune organoid model reveals IL-7 function in coeliac disease Nature 2024-07-24 [PMID: 39048815] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P26717()