Recombinant Human MBL Protein, CF

When Mannan is coated at 0.1 µg/mL (100 µL/well), Recombinant Human MBL (Catalog # 9086-MB) binds with a typical ED50 of 25-150 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MBL Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Mannan is immobilized at 0.1 µg/mL (100 µL/well), the concentration of Recombinant Human MBL that produces 50% of the optimal binding response is approximately 25-150 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human MBL protein
  Glu21-Ile248
• Accession #: CAB56121
• N-terminal Sequence: Glu21
• Protein/Peptide Type: Recombinant Proteins
• Gene: MBL2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 31-36 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MBL Protein, CF

• COLEC1
• COLEC1collectin-1
• Collectin-1
• HSMBPC
• Mannan-binding protein
• mannose-binding lectin (protein C) 2, soluble (opsonic defect)
• mannose-binding lectin (protein C) 2, soluble
• Mannose-binding lectin
• mannose-binding protein C
• MBL
• MBL2
• MBL2D
• MBLmannan-binding lectin
• MBP
• MBP1
• MBP1mannose-binding lectin 2, soluble (opsonic defect)
• MBP-C
• MGC116832
• MGC116833

Background

Human mannose/mannan-binding lectin (MBL) is a 25-30 kDa secreted glycoprotein in the collectin family of pattern-recognition molecules (1, 2). Mature human MBL contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates (3). Human MBL shares 61% amino acid sequence identity with mouse and rat MBL and carries variable post-translation modifications including O-glycosylation and proline and lysine hydroxylation (4). Its collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer (4, 5). MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates (5). Tetrameric complexes of MBL show greater carbohydrate binding capacity compared to the trimers (5). MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3 (5-8). Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria (2). In addition, MBL binds to the scavenger receptor CD91 which mediates the clearance of apoptotic material (9).

1. Scorza, M. et al. (2015) Clin. Chim. Acta 451:78.
2. Matsushita, M. et al. (2013) Arch. Immunol. Ther. Exp. (Warsz.) 61:273.
3. Ezekowitz, R.A.B. et al. (1988) J. Exp. Med. 167:1034.
4. Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
5. Teillet, F. et al. (2005) J. Immunol. 174:2870.
6. Moller-Kristensen, M. et al. (2007) Int. Immunol. 19:141.
7. Thiel, S. et al. (2000) J. Immunol. 165:878.
8. Vorup-Jensen, T. et al. (2000) J. Immunol. 165:2093.
9. Duus, K. et al. (2010) FEBS J. 277:4956.

Publications for MBL (9086-MB)(8)

Publications using 9086-MB

• Dellière, S;Chauvin, C;Wong, SSW;Gressler, M;Possetti, V;Parente, R;Fontaine, T;Krüger, T;Kniemeyer, O;Bayry, J;Carvalho, A;Brakhage, AA;Inforzato, A;Latgé, JP;Aimanianda, V; Interplay between host humoral pattern recognition molecules controls undue immune responses against Aspergillus fumigatus Nature communications 2024-08-14 [PMID: 39138196] (ELISA Capture, Fungus - Aspergillus fumigatus)
• C De Marco V, HL Jewhurst, J Dobó, P Gál, JP Dalton, K Cwiklinski Fasciola hepatica is refractory to complement killing by preventing attachment of mannose binding lectin (MBL) and inhibiting MBL-associated serine proteases (MASPs) with serpins PloS Pathogens, 2022-01-10;18(1):e1010226. 2022-01-10 [PMID: 35007288] (Bioassay, Fasciola hepatica)
• J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019-07-25;14(7):e0219480. 2019-07-25 [PMID: 31344060] (Bioassay, Human)
• J Coumou, A Wagemakers, S Narasimhan, TJ Schuijt, JI Ersoz, A Oei, OJ de Boer, JJTH Roelofs, E Fikrig, JW Hovius The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato Sci Rep, 2019-02-05;9(1):1431. 2019-02-05 [PMID: 30723261] (Bioassay, Parasite - Borrelia burgdorferi)
• F Martini, B Eckmair, S Štefani?, C Jin, M Garg, S Yan, C Jiménez-Ca, A Hykollari, C Neupert, L Venco, D Varón Silv, IBH Wilson, K Paschinger Highly modified and immunoactive N-glycans of the canine heartworm Nat Commun, 2019-01-08;10(1):75. 2019-01-08 [PMID: 30622255] (Binding Assay)
• NM Hammad, NE El Badawy, HA Ghramh, LM Al Kady Mannose-Binding Lectin: A Potential Therapeutic Candidate against Candida Infection Biomed Res Int, 2018-05-02;2018(0):2813737. 2018-05-02 [PMID: 29854737] (Bioassay, Yeast)
• BM Gunn, JE Jones, RS Shabman, AC Whitmore, S Sarkar, LK Blevins, TE Morrison, MT Heise Ross River virus envelope glycans contribute to disease through activation of the host complement system Virology, 2018-01-08;515(0):250-260. 2018-01-08 [PMID: 29324290] (Bioassay, Hamster)
• JC Brazil, R Sumagin, SR Stowell, G Lee, NA Louis, RD Cummings, CA Parkos Expression of Lewis-a glycans on polymorphonuclear leukocytes augments function by increasing transmigration J. Leukoc. Biol., 2017-06-09;0(0):. 2017-06-09 [PMID: 28600306] (Bioassay, Human)

Bioinformatics

• Gene Symbol: MBL2
• Uniprot:
  • CAB56121()