Recombinant Human MBL Protein, CF

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When Mannan is coated at 0.1 µg/mL (100 µL/well), Recombinant Human MBL (Catalog # 9086-MB) binds with a typical ED50 of 25-150 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human MBL Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Mannan is immobilized at 0.1 µg/mL (100 µL/well), the concentration of Recombinant Human MBL that produces 50% of the optimal binding response is approximately 25-150 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human MBL protein
Glu21-Ile248
Accession #
N-terminal Sequence
Glu21
Protein/Peptide Type
Recombinant Proteins
Gene
MBL2
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
31-36 kDa, reducing conditions
Publications
Read Publications using
9086-MB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MBL Protein, CF

  • COLEC1
  • COLEC1collectin-1
  • Collectin-1
  • HSMBPC
  • Mannan-binding protein
  • mannose-binding lectin (protein C) 2, soluble (opsonic defect)
  • mannose-binding lectin (protein C) 2, soluble
  • Mannose-binding lectin
  • mannose-binding protein C
  • MBL
  • MBL2
  • MBL2D
  • MBLmannan-binding lectin
  • MBP
  • MBP1
  • MBP1mannose-binding lectin 2, soluble (opsonic defect)
  • MBP-C
  • MGC116832
  • MGC116833

Background

Human mannose/mannan-binding lectin (MBL) is a 25-30 kDa secreted glycoprotein in the collectin family of pattern-recognition molecules (1, 2). Mature human MBL contains a cysteine-rich region, a collagen-like segment, and a C-type lectin domain that binds to neutral bacterial carbohydrates (3). Human MBL shares 61% amino acid sequence identity with mouse and rat MBL and carries variable post-translation modifications including O-glycosylation and proline and lysine hydroxylation (4). Its collagen-like region mediates MBL association into disulfide-stabilized trimers which further associate into complexes containing three or four copies of the basic trimer (4, 5). MBL is secreted by hepatocytes and opsonizes bacteria through interactions with microbial carbohydrates (5). Tetrameric complexes of MBL show greater carbohydrate binding capacity compared to the trimers (5). MBL multimers can associate with the serine proteases MASP-1, -2, and -3 and promote their cleavage of Complement Component C3 (5-8). Proteolytic cleavage of C3 triggers activation of the complement system and formation of the membrane attack complex, leading to destruction of opsonized bacteria (2). In addition, MBL binds to the scavenger receptor CD91 which mediates the clearance of apoptotic material (9).
  1. Scorza, M. et al. (2015) Clin. Chim. Acta 451:78.
  2. Matsushita, M. et al. (2013) Arch. Immunol. Ther. Exp. (Warsz.) 61:273.
  3. Ezekowitz, R.A.B. et al. (1988) J. Exp. Med. 167:1034.
  4. Jensen, P.H. et al. (2005) J. Biol. Chem. 280:11043.
  5. Teillet, F. et al. (2005) J. Immunol. 174:2870.
  6. Moller-Kristensen, M. et al. (2007) Int. Immunol. 19:141.
  7. Thiel, S. et al. (2000) J. Immunol. 165:878.
  8. Vorup-Jensen, T. et al. (2000) J. Immunol. 165:2093.
  9. Duus, K. et al. (2010) FEBS J. 277:4956.

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Publications for MBL (9086-MB)(6)

We have publications tested in 5 confirmed species: Human, Hamster, Parasite - Borrelia burgdorferi, Parasite - Dirofilaria immitis (Heartworm), Yeast.

We have publications tested in 2 applications: Binding Assay, Bioassay.


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Binding Assay
(1)
Bioassay
(5)
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Filter By Species
Human
(2)
Hamster
(1)
Parasite - Borrelia burgdorferi
(1)
Parasite - Dirofilaria immitis (Heartworm)
(1)
Yeast
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 9086-MB Applications Species
J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019;14(7):e0219480. 2019 [PMID: 31344060] (Bioassay, Human) Bioassay Human
J Coumou, A Wagemakers, S Narasimhan, TJ Schuijt, JI Ersoz, A Oei, OJ de Boer, JJTH Roelofs, E Fikrig, JW Hovius The role of Mannose Binding Lectin in the immune response against Borrelia burgdorferi sensu lato Sci Rep, 2019;9(1):1431. 2019 [PMID: 30723261] (Bioassay, Parasite - Borrelia burgdorferi) Bioassay Parasite - Borrelia burgdorferi
F Martini, B Eckmair, S Štefani?, C Jin, M Garg, S Yan, C Jiménez-Ca, A Hykollari, C Neupert, L Venco, D Varón Silv, IBH Wilson, K Paschinger Highly modified and immunoactive N-glycans of the canine heartworm Nat Commun, 2019;10(1):75. 2019 [PMID: 30622255] (Binding Assay, Parasite - Dirofilaria immitis (Heartworm)) Binding Assay Parasite - Dirofilaria immitis (Heartworm)
NM Hammad, NE El Badawy, HA Ghramh, LM Al Kady Mannose-Binding Lectin: A Potential Therapeutic Candidate against Candida Infection Biomed Res Int, 2018;2018(0):2813737. 2018 [PMID: 29854737] (Bioassay, Yeast) Bioassay Yeast
BM Gunn, JE Jones, RS Shabman, AC Whitmore, S Sarkar, LK Blevins, TE Morrison, MT Heise Ross River virus envelope glycans contribute to disease through activation of the host complement system Virology, 2018;515(0):250-260. 2018 [PMID: 29324290] (Bioassay, Hamster) Bioassay Hamster
JC Brazil, R Sumagin, SR Stowell, G Lee, NA Louis, RD Cummings, CA Parkos Expression of Lewis-a glycans on polymorphonuclear leukocytes augments function by increasing transmigration J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28600306] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol MBL2
Uniprot
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