Recombinant Human LRPAP Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human LRPAP Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized human LRPAP at 0.5 µg/mL can bind rmVLDLR (Catalog # 2258-VL) with an apparent K_D <0.25 nM.
• Source: E. coli-derived human LRPAP protein
  Tyr35-Leu357, with an N-terminal Met and a C-terminal 6-His tag
• Accession #: P30533
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: LRPAP1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 38.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRPAP Protein, CF

• A2MRAP
• A2MRAPMRAP
• A2RAP
• alpha-2-macroglobulin receptor-associated protein 1
• alpha-2-macroglobulin receptor-associated protein
• alpha-2-MRAP
• HBP44
• lipoprotein receptor associated protein
• low density lipoprotein receptor-related protein associated protein 1
• Low density lipoprotein receptor-related protein-associated protein 1
• low density lipoprotein-related protein-associated protein 1(alpha-2-macroglobulin receptor-associated protein 1)
• LRPAP
• LRPAP1
• MGC138272
• RAP

Background

LRPAP (LDL receptor-related protein-associated protein 1; also named RAP), is a ubiquitously expressed 39 kDa chaperone for LDL receptor family proteins (1, 2). Mature human LRPAP shares 77% amino acid sequence identity with mouse and rat LRPAP. It is organized into three domains of comparable length. Domains D2 and D3 interact with each other, while D1 is independent (3). The D1 domain contains a low affinity binding site for LRP, and the associated D2 and D3 domains bind LRP with high affinity (4). The majority of LRPAP is localized in the endoplasmic reticulum and Golgi (5). LRPAP prevents the premature interaction of LRP, LRP2/megalin, and VLDLR with their coexpressed ligands, thereby promoting proper receptor folding and export from the ER (6 - 8). Protonation of conserved histidine residues within the D3 domain induces the separation of LRPAP and LRP in the relatively acidic Golgi (9). LRPAP, which contains a C-terminal HNEL motif, can then recycle to the ER (9). A minor amount of LRPAP remains associated with LRP and can modulate receptor activity on the cell surface (5). Exogenously applied LRPAP competitively inhibits LDL receptor family binding and uptake of activated alpha 2-macroglobulin, apoB100- or apoE-enriched LDL and VLDL particles, cholesteryl esters, and complexes of PAI-1 with either tPA or uPA (10 - 14).

1. Strickland, D.K. et al. (1991) J. Biol. Chem. 266:13364.
2. Bu, G. (2001) Int. Rev. Cytol. 209:79.
3. Lazic, A. et al. (2003) Biochemistry 42:14913.
4. Lazic, A. et al. (2006) Arch. Biochem. Biophys. 450:167.
5. Bu, G. et al. (1994) J. Biol. Chem. 269:29874.
6. Willnow, T.E. et al. (1996) EMBO J. 15:2632.
7. Bu, G. and S. Rennke (1996) J. Biol. Chem. 271:22218.
8. Obermoeller, L.M. et al. (1997) J. Biol. Chem. 272:10761.
9. Lee, D. et al. (2006) Mol. Cell 22:423.
10. Williams, S.E. et al. (1992) J. Biol. Chem. 267:9035.
11. Medh, J.D. et al. (1995) J. Biol. Chem. 270:536.
12. Herz, J. et al. (1991) J. Biol. Chem. 266:21232.
13. Mokuno, H. et al. (1994) J. Biol. Chem. 269:13238.
14. Orth, K. et al. (1992) Proc. Natl. Acad. Sci. 89:7422.

Publications for LRPAP (4296-LR)(3)

Publications using 4296-LR

• T Kirabali, S Rigotti, A Siccoli, F Liebsch, A Shobo, C Hock, RM Nitsch, G Multhaup, L Kulic The amyloid-beta degradation intermediate Abeta34 is pericyte-associated and reduced in brain capillaries of patients with Alzheimer&#039;s disease Acta Neuropathol Commun, 2019-12-03;7(1):194. 2019-12-03 [PMID: 31796114] (Bioassay, Human)
• Kurasawa J, Shestopal S, Karnaukhova E, Struble E, Lee T, Sarafanov A Mapping the binding region on the low density lipoprotein receptor for blood coagulation factor VIII. J Biol Chem, 2013-06-10;288(30):22033-41. 2013-06-10 [PMID: 23754288] (Binding Assay, Human)
• Lodge R, Ouellet M, Barat C, Andreani G, Kumar P, Tremblay MJ HIV-1 promotes intake of Leishmania parasites by enhancing phosphatidylserine-mediated, CD91/LRP-1-dependent phagocytosis in human macrophages. PLoS ONE, 2012-03-06;7(3):e32761. 2012-03-06 [PMID: 22412921] (Bioassay, Human)

Bioinformatics

• Gene Symbol: LRPAP1
• Uniprot:
  • P30533()