Recombinant Human LRG1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human LRG1 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When human Cytochrome c is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human LRG1 that produces 50% of the optimal binding response is 3-15 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human LRG1 protein
Val36-Gln347, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Val36
Protein/Peptide Type
Recombinant Proteins
Gene
LRG1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
35.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-60 kDa, reducing conditions
Publications
Read Publications using
7890-LR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRG1 Protein, CF

  • 1300008B03Rik
  • 2310031E04Rik
  • HMFT1766
  • leucine-rich alpha-2-glycoprotein 1
  • leucine-rich alpha-2-glycoprotein
  • LRG1
  • LRGleucine rich alpha 2 glycoprotein

Background

LRG1 (leucine-rich alpha -2-glycoprotein 1) is an approximately 50 kDa secreted glycoprotein present in the plasma and other body fluids (1‑4). The human LRG1 cDNA encodes 347 amino acids (aa) that include a 35 aa signal sequence, eight leucine‑rich repeats (LRR), and an LRRCT (LRR C‑terminal) domain (1). Mature human LRG1 shares 66%, 63%, 75% and 72% aa sequence identity with mouse, rat, canine and porcine LRG1, respectively. LRG1 is up‑regulated during differentiation of neutrophils and present with myeloperoxidase in primary neutrophilic granules (1, 2). It can be produced by hepatocytes as an acute phase protein (5). LRG1 can interact directly with Cytochrome c, and is thought to act as a survival factor for lymphocytes in the presence of extracellular Cytochrome c (3, 6). Serum or plasma LRG1 is reported to be elevated in toxic shock syndrome, cardiac damage (produced by cardiomyocytes and correlating with BNP), autoimmune syndromes such as rheumatoid arthritis and Crohn’s disease, and some cancers such as those in the ovary and biliary tract (4, 7‑10). It is elevated in coronary sinus fluid during ventricular dysfunction and heart failure, and in peritoneal fluid of patients with uterine lyomyomas (8, 11).

  1. O’Donnell, L.C. et al. (2002) J. Leukoc. Biol. 72:478.
  2. Ai, J. et al. (2008) J. Leukoc. Biol. 83:1277.
  3. Cummings, C. et al. (2006) Apoptosis 11:1121.
  4. Weivoda, S. et al. (2008) J. Immunol. Methods 336:22.
  5. Shirai, R. et al. (2009) Biochem. Biophys. Res. Commun. 382:776.
  6. Codina, R. et al. (2010) Apoptosis 15:139.
  7. Serada, S. et al. (2010) Ann. Rheum. Dis. 69:770.
  8. Watson, C.J. et al. (2011) Circ. Heart Fail. 4:188.
  9. Andersen, J.D. et al. (2010) J. Ovarian Res. 3:21.
  10. Sandanayake, N.S. et al. (2011) Br. J. Cancer 105:1370.
  11. Ferrero, S. et al. (2009) Arch. Gynecol. Obstet. 279:365.

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Publications for LRG1 (7890-LR)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, ELISA (standard).


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ELISA (standard)
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Bioinformatics

Gene Symbol LRG1
Uniprot