Recombinant Human LINGO-1 Protein, CF

When BiotinylatedRecombinant Human Nogo Receptor Fc Chimera is present at 2.5 μg/mL, Recombinant Human LINGO‑1 (Catalog # 9737‑LG) binds with anED50 of 0.4‑2.4 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LINGO-1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human Nogo Receptor Fc Chimera is present at 2.5 μg/mL, the concentration of Recombinant Human LINGO-1 that produces 50% of the optimal binding response is 0.4-2.4 μg/mL.
  
• Source: Human embryonic kidney cell, HEK293-derived human LINGO-1 protein
  Thr40-Thr556, with a C-terminal 6-His tag
• Accession #: Q96FE5-1
• N-terminal Sequence: Thr40
• Protein/Peptide Type: Recombinant Proteins
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 82-112 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LINGO-1 Protein, CF

• FLJ14594
• LERN1
• LERN1UNQ201
• leucine rich repeat and Ig domain containing 1
• leucine rich repeat neuronal 6A
• Leucine-rich repeat and immunoglobilin domain-containing protein 1
• Leucine-rich repeat neuronal protein 1
• Leucine-rich repeat neuronal protein 6A
• LINGO1
• LINGO-1
• LRRN6A
• LRRN6Aleucine-rich repeat neuronal 6A
• MGC17422

Background

LINGO-1 (Leucine-rich repeat and immunoglobulin-like domain-containing Nogo receptor-interacting protein 1), also known as LERN1 (Leucin-rich repeat neuronal protein 1) or LRRN6A (Leucine-rich repeat neuronal protein 6A), is a 614 amino acid (aa) transmembrane protein of the leucine-rich repeat (LRR) family, ribonuclease inhibitor subfamily (1). The four known LINGO proteins (LINGO-1, -2, -3 and -4) contain LRR and IgCAM domains in the extracellular portion and share 44‑61% aa identity (2). LINGO-1 is restricted to the nervous system and is concentrated in the brain as a component of the NgR1/p75 and NgR1/Taj (TROY) signaling complexes (3). LINGO-1 negatively regulates neurite outgrowth and myelination. LINGO-1 is highly conserved, showing 99% aa identity between human, mouse and rat (2, 4). LINGO-1 promotes lysosomal degradation of amyloid-beta protein precursor and acts as a key player in Alzheimer's Disease progression (5). It suppresses oligodendrocyte precursor cell and myelin maturation, which leads to neurodegeneration (6). The intracellular LINGO-1 negatively regulates Trk receptors' signaling by modulating their lysosomal degradation (7). Inhibition of LINGO-1 may protect against degeneration of dopamine neurons in Parkinson's disease (8).

1. Mosyak, L. et al. (2006) J. Biol. Chem. 281:36378.
2. Mi, S. et al. (2004) Nat. Neurosci. 7:221.
3. Fernandez-Enright, F. et al. (2014) Transl. Psychiatry. 4:e348.
4. Mi, S. (2008) Cytokine Growth Factor Rev. 19:245.
5. de Laat, R. et al. (2015) Pathobiol. Aging Age Relat. Dis. 5:25796.
6. Foale, S. et al. (2017) Neural. Regen. Res. 12:1247.
7. Meabon, J.S. et al. (2016) Mol. Cell Neurosci. 70:1.
8. Inoue, H. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:14430.

Bioinformatics

• Uniprot:
  • Q96FE5-1()