Recombinant Human LECT2 Protein, CF

Recombinant Human LECT2 (Catalog # 9908-LC) induces MC3T3-E1mouse preosteoblast cell proliferation. The ED50 for this effect is 0.8-4.8 μg/mL.

2 μg/lane of Recombinant Human LECT2 was resolved with SDS-PAGEunder reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 15-17 kDa and 12-14 kDa,respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LECT2 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using MC3T3-E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.8-4.8 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human LECT2 protein
  Gly19-Leu151
• Accession #: O14960
• N-terminal Sequence: Gly19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-17 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LECT2 Protein, CF

• chm2
• chm-II
• Chondromodulin II
• chondromodulin-II
• hLECT2
• LECT2
• LECT-2
• leukocyte cell-derived chemotaxin 2
• leukocyte cell-derived chemotaxin-2
• MGC126628
• MIM1

Background

LECT2 (leukocyte cell-derived chemotaxin-2), also known as Chondromodulin-II, is a neutrophil chemotactic protein predominantly expressed in the liver (1). It was first identified in the heparin-binding components extracted from fetal bovine epiphyseal cartilage (2). Human LECT2 cDNA encodes a 151 amino acid (aa) precursor that includes an 18 aa signal sequence (3). The mature human LECT2 is a 16 kDa secreted hepatic protein that has a putative peptidase-M23 domain (3, 4). Human LECT2 shares 87% and 86% aa sequence identity with mouse and rat LECT2, respectively. LECT2 stimulates the growth and matrix formation of chondrocytes in vitro (2, 5, 6). In MC3T3-E1 cells, it promotes proliferation but inhibits alkaline phosphatase activity (5, 6). In vivo study suggested LECT2 can directly suppress the development of type II collagen antibody–induced arthritis (4). Recent studies have shown that LECT2 is an important regulator of tumor growth during hepatocellular carcinoma development and progression; it inhibits the angiogenic effect of HUVECs in vitro and in vivo (7).

1. Yamagoe, S. et al. (1996) Immunol. Lett. 52:9.
2. Hiraki, Y. et al. (1996) J. Bio. Chem. 271:22657.
3. Slowik, V. and U. Apte (2017) Clin. Transl. Sci. 10:249.
4. Okumura, A. et al. (2013) FEBS Lett. 587:404.
5. Mori, Y. et al. (1997) FEBS Letters 406:310.
6. Shukunami, C. et al. (1999) J. Biochem. 125:436.
7. Chen, C.K. et al. (2016) Sci. Rep. 6:31398.

Bioinformatics

• Uniprot:
  • O14960()