Recombinant Human LAMP Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LAMP Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic hippocampal neurons. Able to significantly enhance neurite outgrowth when immobilized at 10 µg/mL.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human LAMP protein
  

  Human LAMP (Val29 - Asn315) Accession # AAC50569	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: AAC50569
• N-terminal Sequence: Val29
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: LSAMP
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 58.4 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LAMP Fc Chimera Protein, CF

• FLJ34254
• FLJ35396
• IgLON family member 3
• IGLON3FLJ54658
• LAMP
• LAMPFLJ37216
• limbic system-associated membrane protein
• LSAMP

Background

LAMP (limbic system-associated membrane protein) is a member of the IgLON (immunoglobulin LAMP, OBCAM and neurotrimin) subfamily within the Ig superfamily. All IgLON family members are glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecules that are involved in cell-cell recognition and may have a role in mediating selective neuronal growth and axon targeting. LAMP cDNA encodes a 338 amino acid (aa) residues precursor protein containing a 28 aa N-terminal signal peptide, a 23 aa C-terminal propeptide and a 287 aa mature chain with 3 Ig-like C2-type domains and a GPI-anchor attachment site. In the developing brain, LAMP exhibits a specific pattern of expression in the cortical and subcortical limbic areas, which are important in cognition, emotion, memory, and learning. LAMP is also expressed in single layers of the superior colliculus, spinal chord and cerebellum. LAMP promotes adhesion and growth of limbic axons primarily via homophilic interaction and in part by modulating calcium influx through L-type calcium channels in limbic neurons. Heterophilic interactions between LAMP and neurotrimin have also been demonstrated. LAMP has been shown to inhibit the outgrowth of neurotrimin-expressing dorsal root ganglion neurons in a heterophilic manner. Antibody perturbation studies showed that LAMP is necessary for normal circuit formation in the limbic system, including the septo-hippocampal connection and hippocampal mossy fibers. LAMP acts as an attractive guidance signal for the limbic thalamic axons and can induce branch formation, but also acts as a repulsive axon guidance signal for nonlimbic thalamic axons (1 - 5).

1. Pimenta, A.F. et al. (1995) Neuron, 15:287.
2. Zhukareva, V. and P. Levitt (1995) Development 121:1161.
3. Zhukareva, V. et al. (1997) Mol. Cell Neurosci. 10:43.
4. Mann, F. et al. (1998) Journal Neurosci. 18:9409.
5. Gil, O.D. et al. (2002) J. Neurobiol. 51:190.

Bioinformatics

• Gene Symbol: LSAMP
• Uniprot:
  • AAC50569()