Recombinant Human LAIR2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LAIR2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of HT‑29 human colon adenocarcinoma cells. When 5 x 10⁴ cells/well are added to rhLAIR-2 coated plates (50 µg/mL with
  100 µL/well), approximately 40%-60% will adhere after 10 minutes at 37° C.
  Optimal concentration depends on cell type as well as the application or research objectives.
• Source: Mouse myeloma cell line, NS0-derived human LAIR2 protein
  Gln22-Pro152, with a C-terminal 6-His tag
• Accession #: NP_002279
• N-terminal Sequence: No results obtained: Gln22 predicted & Thr37
• Protein/Peptide Type: Recombinant Proteins
• Gene: LAIR2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LAIR2 Protein, CF

• CD306 antigen
• CD306
• CD306leukocyte-associated Ig-like receptor-2
• LAIR2
• LAIR-2
• leukocyte-associated Ig-like receptor 2
• leukocyte-associated immunoglobulin-like receptor 2
• MGC71634

Background

LAIR-2 (leukocyte-associated Ig-like receptor-2; CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR-1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains; (1-3) although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR-2 may compete with LAIR-1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR-2 is truncated at the C terminus, but retains the entire Ig domain (1-3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.

1. Meyaard, L. (2003) J. Biol. Regul. Homeost. Agents 17:330.
2. Meyaard, L. et al. (1999) J. Immunol. 162:5800.
3. Meyaard, L. et al. (1997)  Immunity 7:283.
4. Xu, X.G. et al. (2005) Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 21:553.

Publications for LAIR2 (2665-LR)(1)

Publication using 2665-LR

• Lebbink RJ, van den Berg MC, de Ruiter T, Raynal N, van Roon JA, Lenting PJ, Jin B, Meyaard L The soluble leukocyte-associated Ig-like receptor (LAIR)-2 antagonizes the collagen/LAIR-1 inhibitory immune interaction. J. Immunol., 2008-02-01;180(3):1662-9. 2008-02-01 [PMID: 18209062] (ELISA (Standard))

Bioinformatics

• Gene Symbol: LAIR2
• Uniprot:
  • NP_002279()