Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to bind KG‑1A human acute myelogenous leukemia cells in a flow cytometry assay. When 250 ng of Recombinant Human KIR2DL1/CD158a Fc Chimera is added to 1 x 106 KG-1A cells, >55% of the cells will bind to the protein.
Source
Mouse myeloma cell line, NS0-derived human KIR2DL1/CD158a protein
Human KIR2DL1/CD158a
(His22-Arg242)
Accession # P43626
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
His22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
KIR2DL1
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
72-80 kDa, reducing conditions
Publications
Read Publications using
1844-KR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KIR2DL1/CD158a Fc Chimera Protein, CF

  • CD158 antigen-like family member A
  • CD158a antigen
  • CD158a
  • CD158Ankat1
  • cl-42
  • killer cell immunoglobulin-like receptor 2DL1
  • killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1
  • killer Ig receptor
  • killer inhibitory receptor 2-2-1
  • KIR221
  • KIR2DL1
  • MHC class I NK cell receptor
  • Natural killer-associated transcript 1
  • NKAT
  • NKAT-1
  • NKAT1KIR-K64
  • p58 killer cell inhibitory receptor KIR-K64
  • p58 natural killer cell receptor clones CL-42/47.11
  • p58 NK cell inhibitory receptor NKR-K6,47.11
  • p58 NK receptor CL-42/47.11
  • p58.1 MHC class-I-specific NK receptor
  • p58.1

Background

KIR2DL1 (2DL1, formerly NKAT1, designated CD158a) is a 348 amino acid (aa) type I transmembrane glycoprotein that belongs to the human killer cell Ig-like receptor (KIR) family (1, 2). KIRs are expressed on human CD56dim NK cells and T cell subsets, and regulate effector functions in the innate immune system (1-3). KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails (1-3). Individuals will express varying subsets of inhibiting and activating KIRs with varying polymorphisms (1, 4). Like other inhibiting KIRs, KIR2DL1 has two ITIM domains within its long tail that block activating receptor clustering (2, 5). Within the ECD, KIR2DL1 shares high aa sequence identity (92%) with KIR2DL2. KIR2DL1 targets Lys80-containing HLA-C2 allotypes while KIR2DL2 rather recognizes Ans80 containing HLA-C1 (1, 6-8). Three KIRs proteins together (2DL1-3) recognize and inhibit NK cytotoxicity against cells expressing any HLA-C allotype, allowing self-recognition, but also conferring susceptibility to leukemia (1-3). In primary human NK cell clones, KIR2DL1 regulates the level of MHC I proteins expression required for NK cell inhibition (9).

  1. Purdy, A.K. and Campbell, K.S. (2009) Cancer Biol. Ther. 8:13.
  2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225.
  3. Kulkarni, S. et al. (2008) Sem. Immunol. 20:343.
  4. Middleton, D. and F. Gonzelez (2009) Immunology 129:8.
  5. Abeyweera, T.P. et al. (2011) J. Cell Biol. 192:675.
  6. Moesta, A.K. et al. (2008) J. Immunol. 180:3969.
  7. Boyington, J.C. et al. (2000) Nature 405:537.
  8. Snyder, G.A. et al. (1999) Proc. Natl. Acad. Sci. USA 96:3864.
  9. Almeida, C.R. et al. (2011) PLoS ONE. 6:e24927.

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Bioinformatics

Gene Symbol KIR2DL1
Uniprot