Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein


Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.

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Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein Summary

Details of Functionality
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.
E. coli-derived human IL-36 beta/IL-1F8 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing conditions
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6834-ILB in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein

  • family of interleukin 1-eta
  • FIL1 eta
  • FIL1
  • FIL1-(ETA)
  • FIL1H
  • FILI-(ETA)
  • IL-1 eta
  • IL1-ETA
  • IL1F8 (Canonical product IL-1F8a)
  • IL-1F8 (FIL1-eta)
  • IL1F8
  • IL-1F8
  • IL1H2
  • IL-1H2
  • IL36 beta
  • IL-36 beta
  • IL36B
  • interleukin 1 family, member 8 (eta)
  • interleukin 1, eta
  • Interleukin 36, Beta
  • Interleukin-1 eta
  • interleukin-1 family member 8
  • Interleukin-1 homolog 2
  • Interleukin-1 Superfamily e
  • Interleukin-36 Beta


Interleukin-36 beta (IL-36 beta, previously known as IL-1F8, FIL-1 eta (eta) and IL-1H2, is a member of the IL-1 family of proteins that also includes IL-1 beta, IL-1 alpha, IL-1ra,
IL-18, IL-36Ra/IL-1F5, IL-36 alpha /IL-1F6, IL-37/IL-1F7, IL-36 gamma /IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12  beta -stranded beta -trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta 1 lacks four of the conserved beta -strands common to the IL-1 family (3). Human IL-36 beta 2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).
  1. Garlanda, C. et al. (2013) Immunity 39:1003.
  2. Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
  3. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  4. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  5. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  6. Wang, P. et al. (2005) Cytokine 29:245.
  7. Vigne, S. et al. (2012) Blood 120:3478.
  8. Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
  9. Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
  10. Foster, A.M. et al. (2014) J. Immunol. 192:6053.
  11. Vigne, S. et al. (2011) Blood 118:5813.
  12. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  13. Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.

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Gene Symbol IL36B