<0.10 EU per 1 μg of the protein by the LAL method.
17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE with silver staining.
Reconstitute at 100 μg/mL in PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein
family of interleukin 1-eta
IL1F8 (Canonical product IL-1F8a)
interleukin 1 family, member 8 (eta)
interleukin 1, eta
Interleukin 36, Beta
interleukin-1 family member 8
Interleukin-1 homolog 2
Interleukin-1 Superfamily e
Interleukin-36 beta (IL-36 beta, previously known as IL-1F8, FIL-1 eta (eta) and IL-1H2, is a member of the IL-1 family of proteins that also includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, IL-36Ra/IL-1F5, IL-36 alpha /IL-1F6, IL-37/IL-1F7, IL-36 gamma /IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12 beta -stranded beta -trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta 1 lacks four of the conserved beta -strands common to the IL-1 family (3). Human IL-36 beta 2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).
Garlanda, C. et al. (2013) Immunity 39:1003.
Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
Johnston, A. et al. (2011) J. Immunol. 186:2613.
Wang, P. et al. (2005) Cytokine 29:245.
Vigne, S. et al. (2012) Blood 120:3478.
Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
Foster, A.M. et al. (2014) J. Immunol. 192:6053.
Vigne, S. et al. (2011) Blood 118:5813.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.
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