Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein

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Recombinant Human IL-36 beta/IL-1F8 (Catalog # 6834-ILB) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein Summary

Details of Functionality
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 0.8-4.8 ng/mL.
Source
E. coli-derived human IL-36 beta/IL-1F8 protein
Arg5-Glu157
Accession #
N-terminal Sequence
Arg5
Protein/Peptide Type
Recombinant Proteins
Gene
IL36B
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
6834-ILB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-36 beta/IL-1F8 (aa 5-157) Protein

  • family of interleukin 1-eta
  • FIL1 eta
  • FIL1
  • FIL1-(ETA)
  • FIL1H
  • FILI-(ETA)
  • IL-1 eta
  • IL1-ETA
  • IL1F8 (Canonical product IL-1F8a)
  • IL-1F8 (FIL1-eta)
  • IL1F8
  • IL-1F8
  • IL1H2
  • IL-1H2
  • IL36 beta
  • IL-36 beta
  • IL36B
  • interleukin 1 family, member 8 (eta)
  • interleukin 1, eta
  • Interleukin 36, Beta
  • Interleukin-1 eta
  • interleukin-1 family member 8
  • Interleukin-1 homolog 2
  • Interleukin-1 Superfamily e
  • Interleukin-36 Beta

Background

Interleukin-36 beta (IL-36 beta, previously known as IL-1F8, FIL-1 eta (eta) and IL-1H2, is a member of the IL-1 family of proteins that also includes IL-1 beta, IL-1 alpha, IL-1ra,
IL-18, IL-36Ra/IL-1F5, IL-36 alpha /IL-1F6, IL-37/IL-1F7, IL-36 gamma /IL-1F9, and IL-1F10 (1, 2). IL-1 family cytokines are characterized by a 12  beta -stranded beta -trefoil configuration and share up to 50% amino acid (aa) sequence identity. The 157 aa human IL-36 beta does not have a canonical signal peptide or prosegment and is expressed as two isoforms that differ in their C-terminal 70 aa. IL-36 beta 1 lacks four of the conserved beta -strands common to the IL-1 family (3). Human IL-36 beta 2 shares 62%, 67%, 63% and 59% aa identity with the most similar isoforms of mouse, canine, bovine and equine IL-36 beta, respectively (4). IL-36 beta is expressed by keratinocytes, naïve CD4+ T cells, neurons, and glia (5-7). It is up-regulated in keratinocytes and synovial fibroblasts by inflammatory stimulation and in psoriatic lesions (5, 8, 9). IL-36 beta promotes inflammatory responses by enhancing the activation and Th1 polarization of dendritic cells and T cells (7, 10, 11). It also enhances the production of multiple pro-inflammatory cytokines, chemokines, and anti-bacterial defensin peptides by keratinocytes, synovial fibroblasts, and articular chondrocytes (5, 8-10). IL-36 proteins exert their bioactivity through a receptor complex that contains IL-1 Rrp2 and IL-1 RAcP, and this is antagonized by IL-36Ra which also binds IL-1 Rrp2 (11, 12). The potency of IL-36 beta is increased by cleavage of its first four N-terminal amino acids (13).
  1. Garlanda, C. et al. (2013) Immunity 39:1003.
  2. Gresnigt, M.S. and F.L. van de Veerdonk (2013) Semin. Immunol. 25:458.
  3. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  4. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  5. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  6. Wang, P. et al. (2005) Cytokine 29:245.
  7. Vigne, S. et al. (2012) Blood 120:3478.
  8. Magne, D. et al. (2005) Arthritis Res. Ther. 8:R80.
  9. Carrier, Y. et al. (2011) J. Invest. Dermatol. 131:2428.
  10. Foster, A.M. et al. (2014) J. Immunol. 192:6053.
  11. Vigne, S. et al. (2011) Blood 118:5813.
  12. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  13. Towne, J.E. et al. (2011) J. Biol. Chem. 286:42594.

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Bioinformatics

Gene Symbol IL36B
Uniprot