Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein

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1 μg/lane of Recombinant Human IL‑36 alpha /IL‑1F6 (aa 6-158) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.
Recombinant Human IL-36 alpha/IL-1F6 (Catalog # 6995-IL) induces IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 4-24 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein Summary

Details of Functionality
Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED50 for this effect is 4-24 ng/mL.
Source
E. coli-derived human IL-36 alpha/IL-1F6 protein
Lys6-Phe158
Accession #
N-terminal Sequence
Lys6
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Gene
IL36A
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
16 kDa, reducing conditions
Publications
Read Publications using
6995-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA and Tween® 20 with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in Water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-36 alpha/IL-1F6 (aa 6-158) Protein

  • FIL1 epsilon
  • FIL1
  • FIL1(EPSILON)
  • FIL1E
  • IL-1 epsilon
  • IL1(EPSILON)
  • IL1E
  • IL-1E
  • IL-1F6 (FIL-1-epsilon)
  • IL1F6
  • IL-1F6
  • IL36 alpha
  • IL-36 alpha
  • IL36A
  • interleukin 1 family, member 6 (epsilon)
  • interleukin 1, epsilon
  • Interleukin 36, Alpha
  • Interleukin-1 epsilon
  • interleukin-1 family member 6
  • Interleukin-36 Alpha

Background

Human IL-36 alpha, previously called IL-1F6 and FIL1 epsilon  (family of IL-1 member epsilon), is a member of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, and novel family members IL-36 Ra (IL-1F5), IL-36 beta (IL-1F8), IL-36 gamma (IL-1F9), IL-37 (IL-1F7) and IL‑38 (IL‑1F10) (1‑4). All family members show a 12  beta ‑strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene (1, 2). IL-36 alpha is an 18‑22 kDa, 158 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential from N‑linked glycosylation sites (1‑3). It can be released in response to LPS and the cell ATP‑induced activation of the P2X7 receptor (5). A 120 aa isoform missing aa 1‑38 has been reported (6). Human IL‑36 alpha (aa 6 ‑ 158) shares 57‑68% aa sequence identity with mouse, rabbit, equine and bovine IL‑36 alpha and 27‑57% aa sequence identity with other novel IL‑1 family members. IL‑36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine (1, 3, 7). It is expressed by monocytes, B and T cells (1, 2). The receptor for IL‑36 alpha is a combination of IL‑1 Rrp2 (also called IL1RL2 or IL‑1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (3, 7). IL-36 alpha, beta, and gamma all activate NF-kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL-8 (7). IL-36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL‑36 alpha in skin keratinocytes produces epidermal hyperplasia (7‑9). IL-36 alpha is present in kidney tubule epithelia, and it is highly expressed in intubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis (10).

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  3. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  4. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  5. Martin, U. et al. (2009) J. Immunol. 183:4021.
  6. Entrez Accession # EAW73614.
  7. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  8. Blumberg, H. et al. (2010) J. Immunol. 185:4354.
  9. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  10. Ichii, O. et al. (2010) Lab. Invest. 90:459.

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Bioinformatics

Gene Symbol IL36A
Uniprot