Recombinant Human IL-2R beta Fc/IL-2R gamma Fc Protein, CF

Measured by its binding ability in a functional ELISA. Recombinant Human IL-2R beta Fc/IL-2R gamma Fc Heterodimer Protein (Catalog # 11686-2B) binds to Recombinant Human IL-2 Protein, CF (BT-002) with an ED50 of 0.300-3.00 μg/mL.

2 μg/lane of Recombinant Human IL-2R beta Fc/IL-2R gamma Fc Heterodimer Protein (Catalog # 11686-2B) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 62-68 kDa (IL-2R beta) & 85-93 kDa (IL-2R gamma).

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-2R beta Fc/IL-2R gamma Fc Protein, CF Summary

• Additional Information: Fc Chimera
• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human IL-2R beta Fc/IL-2R gamma Fc Heterodimer binds to Recombinant Human IL-2 Protein, CF (Catalog # BT-002) with an ED₅₀ of 0.300-3.00 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-2 R beta/IL-2 R gamma protein
  

  Human IL-2RB (Ala27-Asp239) Accession # P14784.1	IEGR	Human IgG1 (Glu99-Lys330) (with modifications)
  Human IL-2RG (Leu23-Asn254) Accession # P31785.1	IEGR	Human IgG1 (Glu99-Lys330) (with modifications)
  N-terminus		C-terminus

• N-terminal Sequence: Ala 27 (IL-2R beta) & Leu 23 (IL-2R gamma)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa (IL-2R beta) & 54 kDa (IL-2 R gamma).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-68 kDa (IL-2R beta) & 85-93 kDa (IL-2R gamma)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2R beta Fc/IL-2R gamma Fc Protein, CF

• CD122
• CD132
• CIDX
• High affinity IL-2 receptor subunit beta
• IL15RB
• IL-2 R beta/IL-2 R gamma
• IL-2 receptor subunit beta
• IL-2RG
• IMD4
• IMD63
• P64
• SCIDX

Background

Interleukin-2 receptor subunit beta (IL-2 RB), also known as high affinity IL-2 receptor subunit beta, interleukin-15 receptor subunit beta, p70-75 (p75), and CD122, is a member of the cytokine receptor superfamily that plays a role in T cell-mediated immune responses (1, 2). Human IL-2 RB consists of an extracellular domain (ECD) containing a fibronectin type III domain and a WSXWS motif, a type I transmembrane domain, and a cytoplasmic tail. The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Functional IL-2 receptors can exist in two affinity states on cell surfaces: the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains (2, 3). Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling (4). 

1. Jia, Z. et al. (2019) Int. Immunpharmacol. 74:105716.
2. Zhang, Z. et al. (2019) J. Exp. Med. 216:1311.
3. Zhou, X. et al. (2019) Fish & Shellfish Immunol. 93:641.
4. Fernandez, I. et al. (2019) J. Exp. Med. 216:1255.