Recombinant Human IL-27 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IL-27 Protein, CF Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 1-8 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-27 protein
Human IL-27 EBI-3
(Arg21-Lys229)
Accession # Q14213.2
GSGSSRGGSGSGGSGGGGSKL  Human IL-27 p28
(Phe29-Pro243)
Accession # AAM34498
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg21
Protein/Peptide Type
Recombinant Proteins
Gene
IL27
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60 kDa, reducing conditions
Publications
Read Publications using
2526-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, EDTA and CHAPS with Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-27 Protein, CF

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family (5). Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region (6). The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (7). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (6, 8, 9). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 7). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (10).

  1. Boulay, J-L. et al. (2003) Immunity 19:159. 
  2. Trinchieri, G. et al. (2003) Immunity 19:641. 
  3. Murakami, M. et al. (2004) Growth Factors 22:75. 
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715. 
  5. Devergne, O. et al. (1996) J. Virology 70:1143. 
  6. Pflanz, S. et al. (2002) Immunity 16:779.
  7. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  8. Pflanz, S. et al. (2004) J Immunol 172:2225.
  9. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  10. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl).193:1.

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Publications for IL-27 (2526-IL/CF)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Complex Species Category.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


Filter By Application
Bioassay
(32)
Cell Culture
(4)
In Vivo
(1)
All Applications
Filter By Species
Human
(36)
Mouse
(1)
Complex Species Category
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using 2526-IL/CF Applications Species
K Otterdal, A Berg, AE Michelsen, A Yndestad, S Patel, I Gregersen, B Halvorsen, T Ueland, N Langeland, P Aukrust IL-18 and IL-18 binding protein are related to disease severity and parasitemia during falciparum malaria BMC infectious diseases, 2021;21(1):1073. 2021 [PMID: 34663245] (Bioassay, Human) Bioassay Human
MA Natale, T Minning, MC Albareda, MD Castro Eir, MG Álvarez, B Lococo, G Cesar, G Bertocchi, MJ Elias, MB Caputo, RL Tarleton, SA Laucella Immune exhaustion in chronic Chagas disease: Pro-inflammatory and immunomodulatory action of IL-27 in vitro PloS Neglected Tropical Diseases, 2021;15(6):e0009473. 2021 [PMID: 34061845] (Cell Culture, Human) Cell Culture Human
ML Chen, X Huang, H Wang, C Hegner, Y Liu, J Shang, A Eliason, H Diao, H Park, B Frey, G Wang, SA Mosure, LA Solt, DJ Kojetin, A Rodriguez-, DA Schady, CT Weaver, ME Pipkin, DD Moore, MS Sundrud CAR directs T cell adaptation to bile acids in the small intestine Nature, 2021;0(0):. 2021 [PMID: 33828301] (Bioassay, Mouse) Bioassay Mouse
T Imamichi, S Goswami, X Hu, S Laverdure, J Yang, J Qiu, Q Chen, BT Sherman, W Chang MicroRNA Profiles in Monocyte-Derived Macrophages Generated by Interleukin-27 and Human Serum: Identification of a Novel HIV-Inhibiting and Autophagy-Inducing MicroRNA International Journal of Molecular Sciences, 2021;22(3):. 2021 [PMID: 33525571] (Bioassay, Human) Bioassay Human
S Srinivasac, I Shcherbako, S Langer, L Koepke, A Preising, D Hotter, F Kirchhoff, KMJ Sparrer, G Schotta, D Sauter HIV-1 infection activates endogenous retroviral promoters regulating antiviral gene expression Nucleic Acids Res, 2020;0(0):. 2020 [PMID: 33021676] (Bioassay, Human) Bioassay Human
G Carbotti, A Petretto, E Naschberge, M Stürzl, S Martini, MC Mingari, G Filaci, S Ferrini, M Fabbi Cytokine-Induced Guanylate Binding Protein 1 (GBP1) Release from Human Ovarian Cancer Cells Cancers (Basel), 2020;12(2):. 2020 [PMID: 32093058] (Cell Culture, Human) Cell Culture Human
K Otterdal, A Berg, AE Michelsen, S Patel, I Gregersen, EL Sagen, B Halvorsen, A Yndestad, T Ueland, N Langeland, P Aukrust Plasma levels of interleukin 27 in falciparum malaria is increased independently of co-infection with HIV: potential immune-regulatory role during malaria BMC Infect. Dis., 2020;20(1):65. 2020 [PMID: 31964363] (Cell Culture, Human) Cell Culture Human
X Hu, S Goswami, J Qiu, Q Chen, S Laverdure, BT Sherman, T Imamichi Profiles of Long Non-Coding RNAs and mRNA Expression in Human Macrophages Regulated by Interleukin-27 Int J Mol Sci, 2019;20(24):. 2019 [PMID: 31835347] (Bioassay, Human) Bioassay Human
D Yamasoba, K Sato, T Ichinose, T Imamura, L Koepke, S Joas, E Reith, D Hotter, N Misawa, K Akaki, T Uehata, T Mino, S Miyamoto, T Noda, A Yamashita, DM Standley, F Kirchhoff, D Sauter, Y Koyanagi, O Takeuchi N4BP1 restricts HIV-1 and its inactivation by MALT1 promotes viral reactivation Nat Microbiol, 2019;0(0):. 2019 [PMID: 31133753] (Bioassay, Human) Bioassay Human
C Petes, V Mintsopoul, RL Finnen, BW Banfield, K Gee The effects of CD14 and IL-27 on induction of endotoxin tolerance in human monocytes and macrophages J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30242126] (Human) Human
Show All 38 Publications.

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