Recombinant Human IL-1ra/IL-1F3 Protein

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1 μg/lane of Recombinant Human IL-1ra/IL-1F3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 20 kDa.
Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits IL-1 alpha -dependent cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 5-40 ng/mL in the presence of 50 pg/mL of ...read more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-1ra/IL-1F3 Protein Summary

Details of Functionality
Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 5-40 ng/mL in the presence of 50 pg/mL of rhIL-1 alpha.
Source
E. coli-derived human IL-1ra/IL-1F3 protein
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
IL1RN
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
280-RA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1ra/IL-1F3 Protein

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

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Publications for IL-1ra/IL-1F3/IL1RN (280-RA)(43)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), In Vivo.


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Binding Assay
(1)
Bioassay
(37)
ELISA (Standard)
(2)
In Vivo
(2)
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Human
(33)
Mouse
(1)
Rat
(2)
Bovine
(3)
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Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 280-RA Applications Species
L Messerschm, S Fischer, P Wiedemann, A Bringmann, M Hollborn Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation Mol. Vis., 2019;25(0):329-344. 2019 [PMID: 31341381] (Bioassay, Human) Bioassay Human
A Lindblad, K Persson, I Demirel IL-1RA is part of the inflammasome-regulated immune response in bladder epithelial cells and influences colonization of uropathogenic E. coli Cytokine, 2019;123(0):154772. 2019 [PMID: 31299415] (Bioassay, Human) Bioassay Human
L Samelko, MS Caicedo, J Jacobs, NJ Hallab Transition from metal-DTH resistance to susceptibility is facilitated by NLRP3 inflammasome signaling induced Th17 reactivity: Implications for orthopedic implants PLoS ONE, 2019;14(1):e0210336. 2019 [PMID: 30653583] (Bioassay, Human) Bioassay Human
K Kinnunen, N Piippo, S Loukovaara, M Hytti, K Kaarnirant, A Kauppinen Lysosomal destabilization activates the NLRP3 inflammasome in human umbilical vein endothelial cells (HUVECs) J Cell Commun Signal, 2017;0(0):. 2017 [PMID: 28547650] (Bioassay, Human) Bioassay Human
N Nishijima, T Hirai, K Misato, M Aoyama, E Kuroda, KJ Ishii, K Higashisak, Y Yoshioka, Y Tsutsumi Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific Front Immunol, 2017;8(0):379. 2017 [PMID: 28421077] (Bioassay, Human) Bioassay Human
Y Song, Y Wang, Y Zhang, W Geng, W Liu, Y Gao, S Li, K Wang, X Wu, L Kang, C Yang Advanced glycation end products regulate anabolic and catabolic activities via NLRP3-inflammasome activation in human nucleus pulposus cells J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28224704] (Bioassay, Human) Bioassay Human
Mihai G Netea A Functional Genomics Approach to Understand Variation in Cytokine Production in Humans Cell, 2016;167(4):1099-1110.e14. 2016 [PMID: 27814507] (Bioassay, Human) Bioassay Human
Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammation Sci Rep, 2016;6(0):25171. 2016 [PMID: 27142573] (Bioassay, Human) Bioassay Human
Sun Y, Zhu D, Wang G, Wang D, Zhou H, Liu X, Jiang M, Liao L, Zhou Z, Hu J Pro-Inflammatory Cytokine IL-1beta Up-Regulates CXC Chemokine Receptor 4 via Notch and ERK Signaling Pathways in Tongue Squamous Cell Carcinoma. PLoS ONE, 2015;10(7):e0132677. 2015 [PMID: 26176534] (Bioassay, Human) Bioassay Human
Larena M, Holmgren J, Lebens M, Terrinoni M, Lundgren A Cholera toxin, and the related nontoxic adjuvants mmCT and dmLT, promote human Th17 responses via cyclic AMP-protein kinase A and inflammasome-dependent IL-1 signaling. J Immunol, 2015;194(8):3829-39. 2015 [PMID: 25786687] (Bioassay, Human) Bioassay Human
Show All 43 Publications.

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Bioinformatics

Gene Symbol IL1RN
Uniprot