Recombinant Human IL-17B Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-17B Protein, CF Summary

• Additional Information: A New and Improved rh IL-17B is Now Available! The new protein is mammalian cell expressed!
• Details of Functionality: Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. Tang, Y. et al. (2011) Clin. Exp. Immunol. 166:281. The ED₅₀ for this effect is 0.4-2 μg/mL.
• Source: E. coli-derived human IL-17B protein
  Gln21-Phe180, with an N-terminal Met
• Accession #: Q9UHF5
• N-terminal Sequence: Gln21
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL17B
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in NaH₂PO₄ and NaCl.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17B Protein, CF

• Cytokine Zcyto7
• cytokine-like protein ZCYTO7
• IL17B
• IL-17B
• IL-17Binterleukin-17 beta
• IL20
• IL-20interleukin-17B
• interleukin 17B
• interleukin 20
• Interleukin-20
• MGC138900
• MGC138901
• neuronal interleukin-17 related factor
• Neuronal interleukin-17-related factor
• NIRF
• ZCYTO7interleukin-20

Background

Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17B shares 90% and 91% amino acid sequence identity with mouse and rat IL-17B, respectively (3, 4). IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine (3-5). In the skeletal system, IL-17B is expressed in bone collars surrounding primary ossification centers as well as in epiphyseal plate chondrocytes during development and after bone fracture (6, 7). IL-17B is up-regulated in arthritic cartilage where it exacerbates the severity of disease (8). It is also up-regulated in aggressive breast cancer cell lines, and its exaggerated signaling through IL-17RB promotes tumorigenicity (9). It inhibits the adhesion of vascular endothelial cells to Matrigel and enhances their migration (10).

1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
4. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
5. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
6. Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89.
7. You, Z. et al. (2005) Biochem. Biophys. Res. Commun. 326:624.
8. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
9. Huang, C.-K. et al. (2013) Oncogene July 15 Epub.
10. Sanders, A.J. et al. (2010) J. Oncol. 2010:817375.

Publications for IL-17B (1248-IB/CF)(3)

Publications using 1248-IB/CF

• G Xiao, R Kumar, Y Komuro, J Burguet, V Kakarla, I Azizkhania, SA Sheth, CK Williams, XR Zhang, M Macknicki, A Brumm, R Kawaguchi, P Mai, N Kaneko, HV Vinters, ST Carmichael, LA Havton, C DeCarli, JD Hinman IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury Cell Reports, 2022-12-20;41(12):111848. 2022-12-20 [PMID: 36543124] (Bioassay, Human)
• Q Bie, H Song, X Chen, X Yang, S Shi, L Zhang, R Zhao, L Wei, B Zhang, H Xiong, B Zhang IL-17B/IL-17RB signaling cascade contributes to self-renewal and tumorigenesis of cancer stem cells by regulating Beclin-1 ubiquitination Oncogene, 2021-03-01;0(0):. 2021-03-01 [PMID: 33649532] (Bioassay, Human)
• Goldstein RH, Reagan MR, Anderson K, Kaplan DL, Rosenblatt M Human bone marrow-derived MSCs can home to orthotopic breast cancer tumors and promote bone metastasis. Cancer Res., 2010-12-15;70(24):10044-50. 2010-12-15 [PMID: 21159629] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL17B
• Uniprot:
  • Q9UHF5()