Recombinant Human IL-12/IL-23 p40 Subunit Protein

When Recombinant Human IL-12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-12/IL-23 p40 Subunit (Catalog # 309-IL) binds with an ED50 of 1-6 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IL-12/IL-23 p40 Subunit Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑12 R beta 1 Fc Chimera (Catalog # 839-B1) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human IL‑12/IL‑23 p40 Subunit that produces 50% of the optimal binding response is 1-6 ng/mL.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-12/IL-23 p40 protein
  Ile23-Ser328
• Accession #: P29460
• N-terminal Sequence: Ile23
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL12B
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-12/IL-23 p40 Subunit Protein

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFN-gamma and TNF production. IL-12 is a disulfide-linked, 70 kDa (p70) heterodimeric glycoprotein composed of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Publications for IL-12/IL-23 p40 (309-IL)(6)

Publications using 309-IL

• S Mondal, M Kundu, M Jana, A Roy, SB Rangasamy, KK Modi, J Wallace, YA Albalawi, R Balabanov, K Pahan IL-12 p40 monomer is different from other IL-12 family members to selectively inhibit IL-12R&amp;beta1 internalization and suppress EAE Proc. Natl. Acad. Sci. U.S.A., 2020-08-19;117(35):21557-21567. 2020-08-19 [PMID: 32817415] (Bioassay, Mouse)
• P Garnier, R Mummery, MJ Forster, B Mulloy, RV Gibbs, CC Rider The localisation of the heparin binding sites of human and murine interleukin-12 within the carboxyterminal domain of the P40 subunit Cytokine, 2018-05-10;110(0):159-168. 2018-05-10 [PMID: 29753267] (Bioassay, Human)
• Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly. J Immunol, 2014-05-12;192(12):6028-36. 2014-05-12 [PMID: 24821971] (Bioassay, Mouse)
• Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production. J. Allergy Clin. Immunol., 2006-06-21;118(2):354-60. 2006-06-21 [PMID: 16890758] (Bioassay, Human)
• Vaknin-Dembinsky A, Balashov K, Weiner HL IL-23 is increased in dendritic cells in multiple sclerosis and down-regulation of IL-23 by antisense oligos increases dendritic cell IL-10 production. J. Immunol., 2006-06-15;176(12):7768-74. 2006-06-15 [PMID: 16751425] (ELISA (Specificity Testing))
• Yoshikawa H, Sato K, Edahiro S, Furukawa Y, Maruta T, Iwasa K, Watanabe H, Takaoka S, Suzuki Y, Takamori M, Yamada M Elevation of IL-12 p40 and its antibody in myasthenia gravis with thymoma. J. Neuroimmunol., 2006-03-29;175(1):169-75. 2006-03-29 [PMID: 16574246] (ELISA (Capture), Human)

Bioinformatics

• Gene Symbol: IL12B
• Uniprot:
  • P29460()