Recombinant Human IL-1 RII Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price

Recombinant Human IL-1 RII Protein Summary

Additional Information
A Newer rh IL-1 RII is Available! ~3 fold better activity; Lower endotoxin specification; CHO expressed; Fc Chimera
Details of Functionality
Measured by its ability to inhibit IL-1 beta -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. Approximately 1-5 µg/mL of IL- sRII will inhibit 50% of the biological response due to 50 pg/mL of recombinant human IL-1 beta.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-1 RII protein
Phe14-Glu343
Accession #
N-terminal Sequence
Phe14
Protein/Peptide Type
Recombinant Proteins
Gene
IL1R2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40 kDa, reducing conditions
Publications
Read Publications using
263-2R in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 RII Protein

  • beta
  • CD121b antigen
  • CD121b
  • IL-1 R beta
  • IL-1 RII
  • IL1R2
  • IL1RBCD121 antigen-like family member B
  • IL-1R-beta
  • IL1RII
  • IL-1RII
  • IL-1RT2
  • IL-1RT-2
  • interleukin 1 receptor, type II
  • Interleukin-1 receptor beta
  • MGC47725

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

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Publications for IL-1 RII (263-2R)(4)

We have publications tested in 2 confirmed species: Complex Species Category, N/A.

We have publications tested in 4 applications: Antibody Absorption, ELISA (Standard), In Vivo, Surface Plasmon Resonance.


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Antibody Absorption
(1)
ELISA (Standard)
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
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Filter By Species
Complex Species Category
(1)
N/A
(3)
All Species

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Bioinformatics

Gene Symbol IL1R2
Uniprot