Recombinant Human IGF-I R/IGF1R Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IGF-I R/IGF1R Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human IGF-I R/IGF1R (Catalog # 391-GR) binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1).
• Source: Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
  Met1-Asn932
• Accession #: P08069
• N-terminal Sequence: Glu31 and Asp741
• Protein/Peptide Type: Recombinant Proteins
• Gene: IGF1R
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 102.9 kDa (single chain); 81.0 kDa ( alpha chain) & 21.9 kDa ( beta chain).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 160 kDa, 125 kDa, 50 kDa & 40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I R/IGF1R Protein, CF

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta  subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Publications for IGF-I R/IGF1R (391-GR)(10)

Publications using 391-GR

• Kim, DY;Kandalaft, H;Lowden, MJ;Yang, Q;Rossotti, MA;Robotham, A;Kelly, JF;Hussack, G;Schrag, JD;Henry, KA;Tanha, J; Sequence tolerance of immunoglobulin variable domain framework regions to non-canonical intradomain disulfide linkages The Journal of biological chemistry 2023-09-22 [PMID: 37742917] (Surface Plasmon Resonance, Human)
• Jung-Won Shin, Sungwon An, Dongin Kim, Hyunjoo Kim, Jinhyung Ahn, Jaehyun Eom et al. Grabody B, an IGF1 receptor-based shuttle, mediates efficient delivery of biologics across the blood-brain barrier Cell Reports Methods 2022-11-21 [PMID: 36452865] (Bioassay, Human)
• A Yogi, G Hussack, H van Faasse, AS Haqqani, CE Delaney, E Brunette, JK Sandhu, M Hewitt, T Sulea, K Kemmerich, DB Stanimirov Brain Delivery of IGF1R5, a Single-Domain Antibody Targeting Insulin-like Growth Factor-1 Receptor Pharmaceutics, 2022-07-12;14(7):. 2022-07-12 [PMID: 35890347] (SPR, Human)
• J Sheff, P Wang, P Xu, M Arbour, L Masson, H van Faasse, G Hussack, K Kemmerich, E Brunette, D Stanimirov, JJ Hill, J Kelly, F Ni Defining the epitope of a blood-brain barrier crossing single domain antibody specific for the type 1 insulin-like growth factor receptor Scientific Reports, 2021-02-19;11(1):4284. 2021-02-19 [PMID: 33608571] (Surface Plasmon Resonance, Human)
• NO Yunn, M Park, S Park, J Lee, J Noh, E Shin, SH Ryu A hotspot for enhancing insulin receptor activation revealed by a conformation-specific allosteric aptamer Nucleic Acids Research, 2021-01-25;49(2):700-712. 2021-01-25 [PMID: 33410883] (Bioassay, Human)
• VR Solomon, E Alizadeh, W Bernhard, A Makhlouf, SV Hartimath, W Hill, A El-Sayed, K Barreto, CR Geyer, H Fonge Development and preclinical evaluation of cixutumumab drug conjugates in a model of insulin growth factor receptor I (IGF-1R) positive cancer Sci Rep, 2020-10-29;10(1):18549. 2020-10-29 [PMID: 33122707] (Bioassay, Human)
• B Akla, M Broussas, N Loukili, A Robert, C Beau-Larvo, M Malissard, N Boute, T Champion, JF Haeuw, A Beck, M Perez, C Dreyfus, M Pavlyuk, E Chetaille, N Corvaia Efficacy of the Antibody-Drug Conjugate W0101 in Preclinical Models of IGF-1 Receptor Overexpressing Solid Tumors Mol. Cancer Ther., 2019-10-08;0(0):. 2019-10-08 [PMID: 31594825] (ELISA, In Vivo, Human, Mouse)
• DM Cedano Pri, Y Cheng, CC Chang, J Yu, YK Takada, Y Takada Direct integrin binding to insulin-like growth factor-2 through the C-domain is required for insulin-like growth factor receptor type 1 (IGF1R) signaling PLoS ONE, 2017-09-05;12(9):e0184285. 2017-09-05 [PMID: 28873464] (ELISA, Human)
• A Rational Engineering Strategy for Designing Protein A-Binding Camelid Single-Domain Antibodies PLoS ONE, 2016-09-15;11(9):e0163113. 2016-09-15 [PMID: 27631624] (Surface Plasmon Resonance)
• Back K, Arnqvist HJ Changes in insulin and IGF-I receptor expression during differentiation of human preadipocytes. Growth Horm. IGF Res., 2008-08-09;19(2):101-11. 2008-08-09 [PMID: 18693051] (ELISA (Standard))

Bioinformatics

• Gene Symbol: IGF1R
• Uniprot:
  • P08069()