Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

Recombinant Human IGF1R/C-His Protein (Catalog # 305-GR) has a molecular weight (MW) of 296.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterotetramer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IGF-I R/IGF1R (Catalog # 305-GR) binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) with an ED 50 of 2.50-30.0 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein Met1-Asn932 with a C-terminal 10-His tag
• Accession #: P08069
• N-terminal Sequence: Glu31 and Asp741
• Protein/Peptide Type: Recombinant Proteins
• Gene: IGF1R
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150 kDa, 120 kDa and 48 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in NaH 2 PO 4 NaCl, EDTA and Tween®-20 with Trehalose.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

1. Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.

Publications for IGF-I R/IGF1R (305-GR)(2)

Publications using 305-GR

• Xu , Lihui, Kohli , Neeraj, Rennard , Rachel, Jiao , Yang, Razlog , Maja, Zhang , Kathy, Baum , Jason, Johnson , Bryan, Tang , Jian, Schoeberl , Birgit, Fitzgerald , Jonathan, Nielsen , Ulrik, Lugovskoy , Alexey A Rapid optimization and prototyping for therapeutic antibody-like molecules. MAbs, 2013-02-07;5(2):237-54. 2013-02-07 [PMID: 23392215] (Bioassay, Yeast)
• Iozzo RV, Buraschi S, Genua M Decorin antagonizes IGF receptor I (IGF-IR) function by interfering with IGF-IR activity and attenuating downstream signaling. J. Biol. Chem., 2011-08-12;286(40):34712-21. 2011-08-12 [PMID: 21840990] (IHC-P, Human)

Bioinformatics

• Gene Symbol: IGF1R
• Uniprot:
  • P08069()