Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

In a functional ELISA, Recombinant Human IGF-I R/IGF1R Fc Chimera Protein (Catalog # 11655-GR) binds to Recombinant Human IGF-I/IGF-1 (291-G1) with an ED50 of 3.00-45.0 ng/mL.

2 μg/lane of (Catalog # 11655-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human IGF-I R/IGF1R Fc Chimera (Catalog # 11655-GR binds to Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) with an ED₅₀ of 3.00-45.0 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IGF-I R/IGF1R protein
  

  Human IGF1R (Glu31-His935) Accession # P08069.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• N-terminal Sequence: Glu31 (apha subunit), Asp741 (beta subunit)
• Structure / Form: Disulfide linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 130 kDa (single chain), 80 kDa (alpha subunit) and 49 kDa (beta subunit).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 160-180 kDa, 110-125 kDa and 70-80 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I R/IGF1R Fc Chimera Protein, CF

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta  subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.