Recombinant Human ICAM-2/CD102 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ICAM-2/CD102 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to rhICAM-2/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >70% will adhere after 1 hour incubation at 37 °C.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human ICAM-2/CD102 protein
  

  Human ICAM-2 (Lys25 - Gln223) Accession # CAA33630	IEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: CAA33630
• N-terminal Sequence: Lys25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: ICAM2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 86 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 0.4 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ICAM-2/CD102 Fc Chimera Protein, CF

• CD102 antigen
• CD102
• ICAM2
• ICAM-2
• intercellular adhesion molecule 2

Background

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.

1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Publications for ICAM-2/CD102 (803-I2)(2)

Publications using 803-I2

• Zhang , Qian, Dove , Christop, Hor , Jyh Lian, Murdock , Heardley, Strauss-Albee , Dara M, Garcia , Jordan A, Mandl , Judith N, Grodick , Rachael, Jing , Huie, Chandler-Brown , Devon B, Lenardo , Timothy, Crawford , Greg, Matthews , Helen F, Freeman , Alexandr, Cornall , Richard, Germain , Ronald N, Mueller , Scott N, Su , Helen C DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity. J Exp Med, 2014-11-24;211(13):2549-66. 2014-11-24 [PMID: 25422492] (Bioassay, Human)
• Del Real G, Jimenez-Baranda S, Mira E, Lacalle RA, Lucas P, Gomez-Mouton C, Alegret M, Pena JM, Rodriguez-Zapata M, Alvarez-Mon M, Martinez-A C, Manes S Statins inhibit HIV-1 infection by down-regulating Rho activity. J. Exp. Med., 2004-08-16;200(4):541-7. 2004-08-16 [PMID: 15314078] (Bioassay, Human)

Bioinformatics

• Gene Symbol: ICAM2
• Uniprot:
  • CAA33630()