Recombinant Human HRP-2 Isoform 1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human HRP-2 Isoform 1 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 6-25 µg/mL on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human HRP-2 protein
  Pro2-Ser671, with a C-terminal 6-His tag
• Accession #: NP_001001520
• N-terminal Sequence: Pro2
• Protein/Peptide Type: Recombinant Proteins
• Gene: HDGFL2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HRP-2 Isoform 1 Protein, CF

• HDGF Like 2
• HDGF2
• HDGF-2
• HDGFL2
• HDGF-Related Protein 2
• HDGFRP2
• Hepatoma-derived growth factor 2
• Hepatoma-Derived Growth Factor Related Protein 2
• Hepatoma-Derived Growth Factor-Related Protein 2
• HRP2
• HRP-2

Background

Hepatoma-derived growth factor (HDGF)-related protein 2 (HRP-2) is a 74 kDa (predicted) member of the HDGF protein family (1 - 5). Similar to the electrophoretic mobility of HRP-3, HRP-2 runs as an anomalously large molecule in SDS-PAGE (4, 5). Members of this gene family have a highly conserved 98 amino acid (aa) sequence at the amino terminus (hath region, for homologous to amino terminus of HDGF) (1). Human HRP-2 is 671 aa in length and has no conserved sequence other than the hath region (1). There is no putative signal peptide sequence for secretion. Located within the hath region is a PWWP domain (aa 5 - 76), named after a conserved Pro-Trp-Trp-Pro motif. The protein also contains a serine-rich region (aa 142 - 269), an arginine-rich region (aa 318 - 391), an HIV-1 Integrase binding domain (IBD) (aa 470-552), a coiled-coil region (aa 521 - 581), and 19 phosphoserine sites that are apparently utilized. There are two isoforms for HRP-2, named 1 and 2. Isoform 2 is missing Ser640 which is found in isoform 1. Human HRP-2 shares 81% and 80% aa sequence identity with mouse and rat HRP-2, respectively. HRP-2 mRNA is highly expressed in testis and skeletal muscle, and is known to exist in neurons, astrocytes and oligodendroglia (1, 5). Like other proteins with a PWWP domain, HRP-2 is imported into the nucleus where it may function as a regulatory protein (1 - 2). The phosphoprotein may also play a role in viral replication, as it has been shown to bind to HIV-Integrase and efficiently reconstitute the in vitro activity of HIV-1 preintegration complexes (2 - 3).

1. Izumoto, Y. et al. (1997) Biochem. Biophys. Res. Commun. 283:26.
2. Cherepanov, P. et al. (2004) J. Biol. Chem. 279:48883
3. Vandegraaff, N. et al. (2006) Virology 346:415.
4. Dietz, F. et al. (2002) Biochem. J. 366:491.
5. El-Tahir, H.M. et al. (2006) BMC Neurosci. Jan 23; 7:6.

Bioinformatics

• Gene Symbol: HDGFL2
• Uniprot:
  • NP_001001520()