Recombinant Human His6-USP1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-USP1 Protein, CF Summary

• Details of Functionality: Recombinant Human USP1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP1 concentration of 0.01-0.5 μM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP1 protein
  Met1 - Leu785 (Gly670Ala & Gly671Ala) with a C-terminal 6-His tag
• Accession #: O94782.1
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP1
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 89 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP1 Protein, CF

• Deubiquitinating Enzyme 1
• EC 3.1.2.15
• EC 3.4.19.12
• hUBP
• ubiquitin carboxyl terminal hydrolase 1
• Ubiquitin Carboxyl-Terminal Hydrolase 1
• ubiquitin specific peptidase 1
• ubiquitin specific processing protease 1
• ubiquitin specific protease 1
• Ubiquitin Thioesterase 1
• Ubiquitin thiolesterase 1
• Ubiquitin-specific-processing protease 1
• UBP
• USP1

Background

USP1 (Ubiquitin-Specific-processing Protease 1, also known as Ubiquitin carboxyl-terminal hydrolase 1) is a deubiquitinating enzyme of the C19 peptidase family and functions as a negative regulator of the Fanconi Anemia pathway. Reported substrates of USP1 include monoubiquitinated FANCD2 and monoubiquitinated PCNA. USP1 plays important roles in DNA damage responses and cancer-related processes, and inhibiting the function of this deubiquitinase sensitizes some cancer cells to chemotherapy. By itself, USP1 is nearly completely inactive and requires a protein binding partner, UAF1 (USP1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) to stimulate its deubiquitinase activity. In addition to the naturally occurring substates listed above, the USP1/UAF1 complex has been demonstrated to cleave K6, K33, and K63-linked polyubiquitin chains. 

1. Cohn, M.A., et al. (2007) Mol. Cell 28:786.
2. Huang, T.T., et al. (2006) Nat. Cell Biol. 8:339.
3. Nijman, S.M.B., et al. (2005) Mol. Cell 17:331.
4. Williams, S.A, et al. (2011) Cell 146:918.

Publications for USP1 (E-564)(1)

Publication using E-564

• M Fottner, AD Brunner, V Bittl, D Horn-Ghetk, A Jussupow, VRI Kaila, A Bremm, K Lang Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase Nat. Chem. Biol., 2019-02-15;15(3):276-284. 2019-02-15 [PMID: 30770915] (Ubiquitination, Human)

Bioinformatics

• Gene Symbol: USP1
• Uniprot:
  • O94782.1()