Recombinant Human HIN-1/SCGB3A1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human HIN-1/SCGB3A1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells. The ED₅₀ for this effect is 0.15-0.75 μg/mL.
• Source: E. coli-derived human HIN-1/SCGB3A1 protein
  Phe21-Gly104
• Accession #: Q96QR1
• N-terminal Sequence: Phe21
• Protein/Peptide Type: Recombinant Proteins
• Gene: SCGB3A1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 6 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HIN-1/SCGB3A1 Protein, CF

• cytokine high in normal-1
• Cytokine HIN-1
• High in normal 1
• HIN1
• HIN-1
• HIN1Pneumo secretory protein 2
• LU105
• PNSP2
• PnSP-2
• PnSP-2Uteroglobin-related protein 2
• SCGB3A1
• secretoglobin family 3A member 1
• secretoglobin, family 3A, member 1
• UGRP2
• UGRP2MGC87867

Background

High in Normal‑1 (HIN‑1), also known as Uteroglobin related protein 2 (UGRP2), is an 8 kDa secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN‑1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2‑5). It is up‑regulated in Clara cells by IL‑4, IL‑13, Oncostatin M, EGF, and TGF‑ alpha and down‑regulated by IFN‑ alpha , beta , and gamma (6‑9). It is secreted into bronchial lavage fluid, saliva, and plasma, and may form disulfide‑linked dimers (10). HIN‑1 binds to the macrophage scavenger receptor MARCO, and to the surface of mammary and bronchial epithelial cells (11, 12). HIN‑1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells (12). The down‑regulation of HIN‑1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 10, 13, 14). Mature human HIN‑1 shares 58% and 62% aa sequence identity with mouse and rat HIN‑1, respectively (4).

1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
2. Porter, D. et al. (2002) Mech. Dev. 114:201.
3. Reynolds, S.D. et al. (2002) Am. J. Respir. Crit. Care Med. 166:1498.
4. Krop, I. et al. (2001) Proc. Natl. Acad. Sci. 98:9796.
5. Niimi, T. et al. (2002) Cytogenet. Genome Res. 97:120.
6. Yamada, A. et al. (2005) J. Immunol. 175:5708.
7. Tomita, T. et al. (2009) Am. J. Respir. Cell Mol. Biol. 40:620.
8. Yamada, A. and S. Kimura (2005) FEBS Lett. 579:2221.
9. Yamada, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:964.
10. Krop, I. et al. (2004) Mol. Cancer Res. 2:489.
11. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
12. Krop, I. et al. (2005) Cancer Res. 65:9659.
13. Krop, I. et al. (2003) Cancer Res. 63:2024.
14. Shigematsu, H. et al. (2005) Int. J. Cancer 113:600.

Bioinformatics

• Gene Symbol: SCGB3A1
• Uniprot:
  • Q96QR1()