Recombinant Human HA-Ubiquitin Protein, CF

Images

 

Product Details

Summary
Product Discontinued
View other related Ubiquitin Peptides and Proteins

Order Details


    • Catalog Number
      U-110
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Recombinant Human HA-Ubiquitin Protein, CF Summary

Details of Functionality
Recombinant Human HA-Ubiquitin can be conjugated to substrate proteins via the subsequent actions of a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human HA-Ubiquitin concentration of 5-50 μM.
Source
E. coli-derived human Ubiquitin protein
Met1 - Gly76 with N-terminal HA (YPYDVPDYA) tag
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
UBB
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
9.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
U-110 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HA-Ubiquitin Protein, CF

  • RPS27A
  • UBA52
  • UBB ubiquitin B
  • UBB
  • UBC
  • Ubiquitin

Background

Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. Ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast Ubiquitin, and 100% aa sequence identity shared between human and mouse Ubiquitin (1). In mammals, four Ubiquitin genes encode for two Ubiquitin-ribosomal fusion proteins and two poly-Ubiquitin proteins. Cleavage of the Ubiquitin precursors by deubiquitinating enzymes gives rise to identical Ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of Ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of Ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a Ubiquitin-activating (E1) enzyme, a Ubiquitin-conjugating (E2) enzyme, and a Ubiquitin ligase (E3). Ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, Ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1) (2,3). In addition to protein degradation, ubiquitination has been shown to mediate a variety of biological processes such as signal transduction, endocytosis, and post-endocytic sorting (4-7).


  1. Sharp, P.M. & W.-H. Li. (1987) Trends Ecol. Evol. 2:328.
  2. Ciechanover, A. et al. (1980 ) Proc. Natl. Acad. Sci. USA 77:1365.
  3. Hershko, A. et al. (1980) Proc. Natl. Acad. Sci. USA 77:1783.
  4. Greene, W. et al. (2012) PLoS Pathog. 8:e1002703.
  5. Tong, X. et al. (2012) J. Biol. Chem. 287:25280.
  6. Wei, W. et al. (2004) Nature 428:194.
  7. Wertz, I.E. et al. (2004) Nature 430:694.
  8. Coux O., et al. (1996) Ann. Rev. Biochem. 65: 801-847.
  9. Ellison M.J and Hochstrasser M. (1991) J. Biol. Chem. 266: 21150-21157.
  10. Finley D., et al. (1994) Mol. Cell. Biol. 14: 5501-5509.
  11. Fritze C.E. and Anderson T.R. (2000) Meth. Enz. 327: 3-16.
  12. Terpe K (2003) Appl. Microbiol. Biotech. 60: 523-533.
  13. Treier M., et al. (1994) Cell. 78: 787-798.
  14. Wilson I.A., et al. (1984) Cell 37:767-778.

Customers Who Viewed This Item Also Viewed...

NB300-221
Species: Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Hu, Mu, Ma-Op, Po, Pm, Rb, Rt, Sh, Ze
Applications: ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Simple Western, Single-Cell Western, WB
NB600-533
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IP, Simple Western, WB
NBP2-33600
Species: Hu, Mu
Applications: ICC/IF, IHC,  IHC-P, WB
NBP1-33527
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, KO, Simple Western, WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NB500-317
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC,  IHC-P, IP, RIA, WB
NB300-263
Species: Bv, Hu, Mu
Applications: IHC,  IHC-P, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gt, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, IP, KD, KO, Simple Western, WB
U-100H
Species: Hu
Applications: EnzAct
MAB2669
Species: Hu
Applications: IHC, Simple Western, WB
NB100-56360
Species: Hu
Applications: WB
H00006908-Q01
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
NBP3-16793
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
NBP3-13522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC,  IHC-P, WB
MAB6609
Species: Hu, Mu
Applications: IHC, WB
NB600-501
Species: Bv, Ca, Ch, Dr(-), Fe, Fi, Gt, Gp, Ha, Hu, Le, Ma, Mu, Po, Pm, Rb, Rt, Sh, Sq, Tr, Ze
Applications: ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr,  IHC-P, IP, KD, KO, Simple Western, WB
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC,  IHC-P, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC,  IHC-P, WB
U-110
Species: Hu
Applications: Enzyme Activity

Publications for Ubiquitin (U-110)(55)

We have publications tested in 4 confirmed species: Human, Drosophila, E. coli, N/A.

We have publications tested in 5 applications: Bioassay, Enzyme Assay, In vitro assay, Ubiquitination, Western Blot.


Filter By Application
Bioassay
(41)
Enzyme Assay
(4)
In vitro assay
(1)
Ubiquitination
(7)
Western Blot
(1)
All Applications
Filter By Species
Human
(28)
Drosophila
(1)
E. coli
(1)
N/A
(8)
All Species
Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using U-110 Applications Species
Yao, Y;Hong, S;Yoshida, S;Swaroop, V;Curtin, B;Inoki, K; The Cullin3-Rbx1-KLHL9 E3 ubiquitin ligase complex ubiquitinates Rheb and supports amino acid-induced mTORC1 activation Cell reports 2024-12-20 [PMID: 39708321] (Bioassay, Human) Bioassay Human
Kochen Rossi, J;Nuevo-Tapioles, C;O'Keefe, RA;Hunkeler, M;Schmoker, AM;Fissore-O'Leary, M;Su, W;Ahearn, IM;Branco, C;Cheong, H;Esposito, D;Clotea, I;Ueberheide, B;Fischer, ES;Philips, MR; The differential interactomes of the KRAS splice variants identify BIRC6 as a ubiquitin ligase for KRAS4A Cell reports 2024-12-19 [PMID: 39705142] (Bioassay, N/A) Bioassay N/A
Liu, G;Huang, K;Liu, S;Xie, Y;Huang, J;Liang, T;Zhang, P; RAD51 plays critical roles in DNMT1-mediated maintenance methylation of genomic DNA by dually regulating the ubiquitin ligase UHRF1 Proceedings of the National Academy of Sciences of the United States of America 2024-12-10 [PMID: 39621902] (Bioassay, N/A) Bioassay N/A
Kim, HS;Roche, B;Bhattacharjee, S;Todeschini, L;Chang, AY;Hammell, C;Verdel, A;Martienssen, RA; Clr4SUV39H1 ubiquitination and non-coding RNA mediate transcriptional silencing of heterochromatin via Swi6 phase separation Nature communications 2024-10-30 [PMID: 39477922] (Bioassay, E. coli) Bioassay E. coli
Wu, S;Hu, C;Zhu, C;Fan, Y;Zhou, J;Xia, X;Shi, K;Zhou, Y;Foyer, CH;Yu, J; The MYC2-PUB22-JAZ4 module plays a crucial role in jasmonate signaling in tomato Molecular plant 2024-02-09 [PMID: 38341757] (Bioassay, N/A) Bioassay N/A
Olazabal-Herrero, A;He, B;Kwon, Y;Gupta, AK;Dutta, A;Huang, Y;Boddu, P;Liang, Z;Liang, F;Teng, Y;Lan, L;Chen, X;Pei, H;Pillai, MM;Sung, P;Kupfer, GM; The FANCI/FANCD2 complex links DNA damage response to R-loop regulation through SRSF1-mediated mRNA export Cell reports 2024-01-23 [PMID: 38165804] (Bioassay, N/A) Bioassay N/A
Liu, M;Wang, Y;Teng, F;Mai, X;Wang, X;Su, MY;Stjepanovic, G; Structure of the DDB1-AMBRA1 E3 ligase receptor complex linked to cell cycle regulation Nature communications 2023-11-22 [PMID: 37993427] (Bioassay, N/A) Bioassay N/A
Xu, Y;Qu, M;He, Y;He, Q;Shen, T;Luo, J;Tan, D;Bao, H;Xu, C;Ji, X;Hu, X;Barkat, MQ;Zeng, LH;Wu, X; Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions The Journal of biological chemistry 2023-10-25 [PMID: 37890777] (Bioassay, N/A) Bioassay N/A
Yamanaka, S;Furihata, H;Yanagihara, Y;Taya, A;Nagasaka, T;Usui, M;Nagaoka, K;Shoya, Y;Nishino, K;Yoshida, S;Kosako, H;Tanokura, M;Miyakawa, T;Imai, Y;Shibata, N;Sawasaki, T; Lenalidomide derivatives and proteolysis-targeting chimeras for controlling neosubstrate degradation Nature communications 2023-08-18 [PMID: 37596276] (In vitro assay) In vitro assay
R Zhou, J Chen, Y Xu, Y Ye, G Zhong, T Chen, L Qiu PRPF19 facilitates colorectal cancer liver metastasis through activation of the Src-YAP1 pathway via K63-linked ubiquitination of MYL9 Cell Death & Disease, 2023-04-08;14(4):258. 2023-04-08 [PMID: 37031206] (Bioassay, Human) Bioassay Human
Show All 55 Publications.

Reviews for Ubiquitin (U-110) (0)

There are no reviews for Ubiquitin (U-110). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Ubiquitin (U-110) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Ubiquitin Products

Blogs on Ubiquitin.

There's an autophagy for that!
By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

Article Review: Glucose-induced transcriptional regulation in cancer
Epigenetic mechanisms have been implicated in many physiological and pathophysiological processes. Among these, histone modifications including methylation, phosphorylation, acetylation and ubiquitination, significantly modify gene expression. In c...  Read full blog post.

PINK1: All work and no fun
The protein PINK1 is a mitochondrial-located serine/threonine kinase (PTK) that maintains organelle function and integrity. It not only protects organelles from cellular stress, but it also uses the selective auto-phagocytosis process for cleaning and...  Read full blog post.

Ubiquitin-Mediated Degradation of Cellular Proteins: The Kiss of Death
Ubiquitin is an abundant and essential cellular 9-kd protein that is conserved across evolution from yeast to humans. Ubiquitin is used by cells as a covalent modifier of other proteins both to activate their function and to target them for degradatio...  Read full blog post.

Using Ubiquitin Antibodies in Various Disease Research
Ubiquitin is a small, highly conserved protein which plays an important role in protein breakdown, covalently bonding to proteins to mark them for proteolytic degradation in a process called ubiquitination. Ubiquitin also binds to inclusion bodies (ac...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

The Latest Research on IBR-type E3 Ubiquitin Ligases
E3 ubiquitin ligases are standards in most antibody catalogs. These proteins are essential to the process of ubiquitination, which is expressed in protein pathways throughout the body and is often linked to disease states. It is widely used as a bioma...  Read full blog post.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human HA-Ubiquitin Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol UBB
Uniprot