Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF Summary

• Additional Information: Will be discontinued when existing inventory is gone.
• Details of Functionality: Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) concentration of 50-200 nM.
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Ubiquitin E1 Enzyme/UBE1L2 protein
  Met1 - Asp1052 with a N-terminal GST (glutathione S-transferase) tag
• Accession #: ABR25253.1
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 148 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES and DTT. See Certificate of Analysis for details.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

• MOP4
• UBA6
• UBEL2
• Ubiquitin E1 Enzyme

Background

Ubiquitin-activating Enzyme 1-like 2 (UBE1L2), also known as Ubiquitin-like Modifier Activating Enzyme 6 (UBA6), is a 1052 amino acid (aa) member of the Ubiquitin-activating (E1) enzyme family with a predicted molecular weight of 118 kDa. The mouse and rat UBE1L2/UBA6 orthologs share 88% aa sequence identity with the human protein. UBE1L2/UBA6 is expressed in vertebrate cells where four isoforms have been identified (1). UBE1L2/UBA6 is an activating enzyme for both Ubiquitin and FAT10 via a similar molecular mechanism but has a higher affinity for FAT10 than for Ubiquitin (2-4). UBE1L2/UBA6 contains an ATP-binding motif and an active site cysteine residue, Cys625 in humans, common to E1 enzymes. It shares 40% aa sequence identity with the related family member Ubiquitin Activating Enzyme/UBE1 (1). Similar to UBE1, UBE1L2/UBA6 can transfer activated Ubiquitin to Ubiquitin-conjugating (E2) enzymes including the UbcH5 isoforms and UbcH7 (5). Unlike UBE1, which can transfer Ubiquitin to the cell cycle-associated E2 enzyme, UbcH3, UBE1L2/UBA6 can transfer Ubiquitin to Use1/UBE2Z, an E2 enzyme that cannot be charged with Ubiquitin by UBE1 (1,5). UBE1L2/UBA6-initiated covalent linkage of FAT10 to protein targets results in proteasomal degradation (2). UBE1L2/UBA6 is essential for embryonic development (3). One isoform, nUBE1L, is highly expressed in human testis and may play a role in spermatogenesis and male fertility (6).

1. Pelzer, C. et al. (2007) J. Biol. Chem. 282:23010.
2. Pelzer, C. & M. Groettrup (2010) Subcell. Biochem. 54:238.
3. Chiu, Y.H. et al. (2007) Mol. Cell 27:1014.
4. Gavin, J.M. et al. (2012) J. Biol. Chem. 287:15512.
5. Jin, J. et al. (2007) Nature 447:1135.
6. Zhu, H. et al. (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36:227.

Publications for Ubiquitin E1 Enzyme/UBE1L2 (E-307)(3)

Publications using E-307

• Yan, W;Zhong, Y;Hu, X;Xu, T;Zhang, Y;Kales, S;Qu, Y;Talley, DC;Baljinnyam, B;LeClair, CA;Simeonov, A;Polster, BM;Huang, R;Ye, Y;Rai, G;Henderson, MJ;Tao, D;Fang, S; Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes Nature communications 2023-08-09 [PMID: 37558718] (Bioassay, N/A)
• AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer Nat. Chem. Biol., 2020-08-17;0(0):. 2020-08-17 [PMID: 32807965] (Bioassay, Human, N/A)
• Xu , Xinsong, Keshwani , Malik, Meyer , Kathleen, Sarikas , Antonio, Taylor , Susan, Pan , Zhen-Qia Identification of the degradation determinants of insulin receptor substrate 1 for signaling cullin-RING E3 ubiquitin ligase 7-mediated ubiquitination. J Biol Chem, 2012-10-08;287(48):40758-66. 2012-10-08 [PMID: 23045529] (Binding Assay)

Bioinformatics

• Uniprot:
  • ABR25253.1()