UBR2 Antibody

Images

 
Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

UBR2 Antibody Summary

Immunogen
Peptide with sequence SCPKSGGDKSRAPT corresponding to internal region according to NP_056070.1.
Epitope
SCPKSGGDKSRAPT
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry-Paraffin 5ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band in rat testis lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199 kDa band according to rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-45243 in the following applications:

Publications
Read Publications using
NBP1-45243 in the following applications:

  • WB
    4 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28740232). Mouse reactivity reported in scientific literature (PMID: 27897407). Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for UBR2 Antibody

  • bA49A4.1
  • C6orf133MGC71112
  • chromosome 6 open reading frame 133
  • dJ242G1.1
  • dJ392M17.3
  • DKFZp686C08114
  • E3 ubiquitin-protein ligase UBR2
  • EC 6.3.2.-
  • KIAA0349FLJ36357
  • N-recognin-2
  • RP3-392M17.3
  • ubiquitin protein ligase E3 component n-recognin 2
  • Ubiquitin-protein ligase E3-alpha-2
  • Ubiquitin-protein ligase E3-alpha-II

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UBR2 Antibody (NBP1-45243)(5)

Review for UBR2 Antibody (NBP1-45243) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-45243:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot UBR2 NBP1-45243
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reviewed by:
Anonymous
WB Mouse 09/06/2017
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Summary

ApplicationWestern Blot
Sample Tested293 cells transfected with UBR2,Mouse myoblasts (C2C12 cell lysate)
SpeciesMouse
LotE151211

Comments

Comments1:1000 dilution in BSA, incubate overnight

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for UBR2 Antibody (NBP1-45243) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/06/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol UBR2
Entrez
Uniprot