UBR2 Antibody

Images

 
Western Blot: UBR2 Antibody [NBP1-45243] - CD9-positive EVs induce catabolic response in C2C12 myotubes. EVs isolated from LCM were subjected to immunoprecipitation (IP) against CD9 with pre-immune IgG as control. ...read more
Immunohistochemistry-Paraffin: UBR2 Antibody [NBP1-45243] - Staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
UBR2 Antibody [NBP1-45243] - Hsp70 and 90-deficient LLC tumor does not induce muscle catabolism in mice. TA of mice derived from b was analyzed by western blotting for markers of catabolic pathways. Data (n=6) were ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

UBR2 Antibody Summary

Immunogen
Peptide with sequence SCPKSGGDKSRAPT corresponding to internal region according to NP_056070.1.
Epitope
SCPKSGGDKSRAPT
Predicted Species
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBR2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 ug/ml
Application Notes
WB: Preliminary experiments gave bands at approx. 150 kDa band in rat testis lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 199 kDa band according to rat NP_001171542.1. The detected band was successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Reviewed Applications
Read 1 Review rated 5
using
NBP1-45243 in the following applications:

Publications
Read Publications using
NBP1-45243 in the following applications:

  • 1 publication
  • WB
    8 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28740232). Mouse reactivity reported in scientific literature (PMID: 27897407).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for UBR2 Antibody

  • bA49A4.1
  • C6orf133MGC71112
  • chromosome 6 open reading frame 133
  • dJ242G1.1
  • dJ392M17.3
  • DKFZp686C08114
  • E3 ubiquitin-protein ligase UBR2
  • EC 6.3.2.-
  • KIAA0349FLJ36357
  • N-recognin-2
  • RP3-392M17.3
  • ubiquitin protein ligase E3 component n-recognin 2
  • Ubiquitin-protein ligase E3-alpha-2
  • Ubiquitin-protein ligase E3-alpha-II

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00197131-M01
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, S-ELISA, WB
NBP1-31302
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
NBP1-98549
Species: Hu
Applications: WB
NBP2-47561
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-15271
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB100-1591
Species: Hu, Mu
Applications: IHC, IHC-P, IP, KD, WB
NBP1-28730
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-553
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP1-88409
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NB100-56346
Species: Hu, Mu, Sh
Applications: ELISA, Flow, IHC, IHC-P, WB
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, WB
NBP1-77685
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-94071
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-76836
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
NBP2-24915
Species: Bv, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP2-21037
Species: Hu
Applications: IHC, IHC-P, WB

Publications for UBR2 Antibody (NBP1-45243)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(1)
WB
(8)
All Applications
Filter By Species
Human
(2)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-45243 Applications Species
Shi X, Yang J, Liu M et al. Circular RNA ANAPC7 inhibits tumor growth and muscle wasting via PHLPP2-AKT-TGF-beta signaling axis in pancreatic cancer Gastroenterology Feb 14 2022 [PMID: 35176309] (WB, Mouse) WB Mouse
Sin, T K, Zhang, G Et al. Cancer-induced muscle wasting requires p38beta MAPK activation of p300. Cancer Res Dec 22 2020 [PMID: 33355181] (ChIP, Human) ChIP Human
Liu Zhelong, Sin Ka Wai Thomas, Ding Hui et al. p38 beta MAPK mediates ULK1-dependent induction of autophagy in skeletal muscle of tumor-bearing mice. Cell Stress 2018 Oct 10 [PMID: 31225455] (WB, Mouse) WB Mouse
Li YP, Sin TK, Zhu JZ, Zhang G. p300 mediates muscle wasting in Lewis lung carcinoma Cancer Res. Jan 31 2019 [PMID: 30705122] (WB, Mouse) WB Mouse
Zhang G, Liu Z, Ding H et al. Tumor induces muscle wasting in mice through releasing extracellular Hsp70 and Hsp90 Nat Commun 2017 Sep 19 [PMID: 28928431] (WB, Mouse) WB Mouse
Cha-Molstad H, Yu JE, Feng Z et al. p62/SQSTM1/Sequestosome-1 is an N-recognin of the N-end rule pathway which modulates autophagosome biogenesis Nat Commun 2017 Jul 24 [PMID: 28740232] (WB, Human) WB Human
Zhang Guohua, Liu Zhelong, Ding Hui et al. Toll-like receptor 4 mediates Lewis lung carcinoma-induced muscle wasting via coordinate activation of protein degradation pathways. Scientific Reports 2017 [PMID: 28536426] (WB, Mouse) WB Mouse
Ding H, Zhang G, Sin KW et al. Activin A induces skeletal muscle catabolism via p38beta mitogen activated protein kinase J Cachexia Sarcopenia Muscle Jul 25 2016 [PMID: 27897407] (WB, Mouse) WB Mouse
Zhang G, Lin RK, Kwon YT, Li YP. Signaling mechanism of tumor cell-induced up-regulation of E3 ubiquitin ligase UBR2 FASEB J 2013 Apr 8 [PMID: 23568773] (WB, Mouse) WB Mouse
Zenker M, Mayerle J, Lerch MM et al. Deficiency of UBR1, a ubiquitin ligase of the N-end rule pathway, causes pancreatic dysfunction, malformations and mental retardation (Johanson-Blizzard syndrome). Nat Genet 2005 Dec [PMID: 16311597]

Review for UBR2 Antibody (NBP1-45243) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-45243:
Filter by Applications
Western Blot
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot UBR2 NBP1-45243
Enlarge
5
reviewed by:
Lidan Zhao
Western Blot Mouse 09/06/2017
View

Summary

ApplicationWestern Blot
Sample Tested293 cells transfected with UBR2,Mouse myoblasts (C2C12 cell lysate)
SpeciesMouse
LotE151211

Comments

Comments1:1000 dilution in BSA, incubate overnight

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for UBR2 Antibody (NBP1-45243) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional UBR2 Products

Bioinformatics Tool for UBR2 Antibody (NBP1-45243)

Discover related pathways, diseases and genes to UBR2 Antibody (NBP1-45243). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UBR2 Antibody (NBP1-45243)

Discover more about diseases related to UBR2 Antibody (NBP1-45243).
 

Pathways for UBR2 Antibody (NBP1-45243)

View related products by pathway.

PTMs for UBR2 Antibody (NBP1-45243)

Learn more about PTMs related to UBR2 Antibody (NBP1-45243).

Blogs on UBR2

There are no specific blogs for UBR2, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Lidan Zhao
09/06/2017
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol UBR2
Entrez
Uniprot