SETDB2 Antibody

Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - Human dermal microvascular endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488. Image submitted by a verified customer review.

Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.

Western Blot: SETDB2 Antibody [NB100-1137] - RL human non-Hodgkin's lymphoma B cell line whole cell lysate (WCL). Single band around correct MW is specific to the target protein SETDB2. Image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - SETDB2 in human induced Pluripotent Stem Cells (iPSCs) using anti-SETDB2 antibody. Image submitted by a verified customer review.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ICC/IF, IP, PEP-ELISA, ChIP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SETDB2 Antibody Summary

• Immunogen: Peptide with sequence GEKNGDAKTFWME-C corresponding to N-Terminus according to NP_114121.2, NP_001153780.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SETDB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 1 - 3 ug/mL
• Application Notes: Approx 85 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 81.9 kDa according to NP_114121.2). Not suitable on mouse heart and rat heart lysates. This SETDB2 antibody is validated for WB, CHIP, ICC/IF IP from verified customer reviews.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SETDB2 Antibody

• C13orf4
• chromosome 13 open reading frame 4
• chronic lymphocytic leukemia deletion region 8
• Chronic lymphocytic leukemia deletion region gene 8 protein
• CLLD8DKFZp761J1217
• DKFZp586I0123
• EC 2.1.1.43
• histone-lysine N-methyltransferase SETDB2
• KMT1FCLLL8
• Lysine N-methyltransferase 1F
• SET domain bifurcated 2
• SET domain, bifurcated 2

Background

Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SETDB2 Antibody (NB100-1137)(2)

Publications using NB100-1137

• Roy A, Ghosh A, Kumar B, Chandran B IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP Elife 2019-11-04 [PMID: 31682228] (ICC/IF, IP, WB, Chemotaxis, Human)
• Mabuchi H, Fujii H, Calin G et al. Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel candidate genes for leukemogenesis at chromosome 13q14, a region commonly deleted in B-cell chronic lymphocytic leukemia. Cancer Res 2001-04-01 [PMID: 11306461]

Reviews for SETDB2 Antibody (NB100-1137) (6)

Average Rating: 4

(Based on 6 reviews)

We have 6 reviews tested in 1 species: Human.

reviewed by: Anandita Ghosh WB Human 07/18/2018

Summary

• Application: Western Blot
• Sample Tested: THP1 cell line
• Species: Human
• Lot: S3G2N16/04/2013

reviewed by: Arunava Roy ChIP Human 06/22/2018

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: B cells
• Species: Human
• Lot: S3G2N16/04/2013

reviewed by: Arunava Roy ICC Human 06/18/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Human Dermal Microvascular Endothelial cells
• Species: Human
• Lot: S3G2N16/04/2013

reviewed by: Arunava Roy IP Human 05/16/2018

Summary

• Application: Immunoprecipitation
• Sample Tested: RL human non-Hodgkin's lymphoma B cell line
• Species: Human
• Lot: S3G2N16/04/2013

reviewed by: Arunava Roy WB Human 01/04/2018

Summary

• Application: Western Blot
• Sample Tested: RL human non-Hodgkin's lymphoma B cell line
• Species: Human
• Lot: S3G2N16/04/2013

reviewed by: Lucy McDermott ICC Human 02/25/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: Induced pluripotent stem cells
• Species: Human

Bioinformatics

• Gene Symbol: SETDB2
• Entrez:
  • 83852(Human)
• Uniprot:
  • NP_114121.(Human)