Recombinant Human FSH alpha/beta Protein, CF

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1 µg/lane of Recombinant Human FSH alpha / beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at20-25 kDa.
Recombinant Human FSH alpha / beta (Catalog # 5925-FS/CF) induces cAMP accumulation in the HEK293 human embryonic kidney cell line transfected with human FSH R. The ED50 for this effect is 25-200 pg/mL.
40 µg of Recombinant Human FSH alpha / beta (blue line) was applied onto a Superdex 200 column. The peak eluted at approximately 44 kDa, form overlaid on the molecular weight markers (gray line), corresponds to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human FSH alpha/beta Protein, CF Summary

Details of Functionality
Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human FSH R. The ED50 for this effect is 25-200 pg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human FSH alpha/beta protein
Human FSH alpha  
Ala25-Ser116
Accession # P01215
Human FSH beta
Cys21-Glu129
Accession # P01225
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala25 (FSH alpha ) & Cys21 (FSH beta )
Structure / Form
Noncovalently-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FSHB
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.2 kDa (FSH alpha ) & 12.4 kDa (FSH beta ).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22-26 kDa, reducing conditions
Publications
Read Publications using
5925-FS/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FSH alpha/beta Protein, CF

  • FSH alpha/beta

Background

Follicle-Stimulating Hormone (FSH) is a 33 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit is also a component of Luteinizing Hormone (LH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with the FSH Receptor (1-3). The approximately 20 kDa alpha subunit of human FSH shares 73% and 72% aa sequence identity with the mouse and rat orthologs, respectively. The approximately 21 kDa human FSH beta subunit shares 85% and 82% aa sequence identity with the mouse and rat orthologs, respectively. FSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, FSH secretion can also be stimulated by activins and inhibited by inhibins (4-6). FSH works in concert with LH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (7). FSH can also regulate estrogen formation by inducing the aromatase enzyme that converts androgen to estradiol (7, 8). In addition, it has been suggested to have a role in postmenopausal bone loss and in the neovascularization surrounding malignant tumors (9-11). In males, FSH is critical for sperm production (12). Mutations in the human FSH and FSHR genes are associated with reduced reproductive functioning in females (2, 11). Additionally, increased production of autoantibodies directed against FSH beta has been suggested to contribute to certain types of female infertility (2). Assisted reproductive technology commonly uses recombinant FSH to induce follicle growth (7).
  1. Fox, K.M. et al. (2001) Mol. Endocrinol. 15:378.
  2. Haller-Kikkatalo, K. et al. (2012) Clin. Dev. Immunol. 2012:762541.
  3. Jiang, X. et al. (2014) Mol. Cell. Endocrinol. 382:424.
  4. Findlay, J.K. (1993) Biol. Reprod. 48:15.
  5. Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
  6. Bernard, D.J. and S. Tran (2013) Biol. Reprod. 88:1.
  7. Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
  8. Erickson, G.F. and A.J. Hsueh (1978) Endocrinology 102:1275.
  9. Sun, L. et al. (2006) Cell 125:247.
  10. Radu, A. et al. (2010) N. Engl. J. Med. 363:1621.
  11. Laan, M. et al. (2012) Curr. Opin. Endocrinol. Diabetes Obes. 19:220.
  12. Franchimont, P. et al. (1975) J. Reprod. Fertil. 44:335.

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Publications for FSH alpha/beta (5925-FS/CF)(3)

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