Recombinant Human Frizzled-9 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Frizzled-9 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an ED₅₀ of 15-90 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Frizzled-9 protein
  

  Human Frizzled-9 (Leu23-Asp185) Accession # O00144	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O00144
• N-terminal Sequence: Leu23
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 54-63 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Frizzled-9 Fc Chimera Protein, CF

• CD349 antigen
• CD349
• frizzled (Drosophila) homolog 9
• frizzled homolog 9 (Drosophila)
• Frizzled9
• Frizzled-9
• fz-9
• FZD3fzE6
• FZD9
• FzE6
• hFz9

Background

Frizzled-9 (Fzd9, previously called Fzd3) belongs to the Frizzled family of seven-transmembrane glycoprotein receptors, which are signaling receptors for the family of secreted Wnt ligands (1-3). Mature human Frizzled-9 consists of a 207 amino acid (aa) N-terminal extracellular domain (ECD), the 7TM region, and a 62 aa C-terminal cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region that binds Wnts and is highly conserved among Frizzleds. Within aa 23-185 of the N-terminal ECD, human Frizzled-9 shares 97% aa identity with mouse and rat Frizzled-9. The human Frizzled-9 gene maps to a large region that is deleted in Williams-Beuren syndrome, a developmental disorder affecting personality and cognition (2). Frizzled-9 mRNA is expressed in the central nervous system and myotomes during development, and it persists in the hippocampus throughout life (2-4). Adults also show expression in heart, testis, and skeletal muscle (2-4). Cellular expression is detected in proliferating neural precursors, B lymphocyte progenitors, differentiating osteoblasts, and developing myotubes of skeletal muscle (4-7). Mice lacking Frizzled-9 exhibit defects in learning, B lymphocyte development, and bone mineralization (4-6, 8). Frizzled-9 can signal through both canonical and non-canonical pathways (6, 9). Its binding to Wnt-7a promotes the activation of PPAR gamma , expression of Cadherin and Sprouty, and reversal of non-small cell lung cancer cell transformation (10-12).

1. Dijksterhuis, J.P. et al. (2014) Br. J. Pharmacol. 171:1195.
2. Wang, Y.K. et al. (1997) Hum. Mol. Genet. 6:465.
3. Wang, Y.K. et al. (1999) Genomics 57:235.
4. Zhao, C. et al. (2005) Development 132:2917.
5. Ranheim, E.A. et al. (2005) Blood 105:2487.
6. Albers, J. et al. (2011) J. Cell Biol. 192:1057.
7. Aviles, E.C. et al. (2014) Front. Cell. Neurosci. 8:110.
8. Heilmann, A. et al. (2013) PLoS One 8:e84232.
9. Karasawa, T. et al. (2002) J. Biol. Chem. 277:37479.
10. Winn, R.A. et al. (2005) J. Biol. Chem. 280:19625.
11. Winn, R.A. et al. (2006) J. Biol. Chem. 281:26943.
12. Tennis, M.A. et al. (2010) Mol. Cancer Res. 8:833.

Publications for Frizzled-9 (9175-FZ)(1)

Publication using 9175-FZ

• R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy Embo Molecular Medicine, 2021-06-09;0(0):e13977. 2021-06-09 [PMID: 34105895] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • O00144()