Recombinant Human FLRT2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human FLRT2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. Recombinant Human FLRT2 immobilized at 5 μg/mL, 100 μL/well, will mediate >50% Neuro‑2A cell adhesion.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human FLRT2 protein
  Cys36-Ser539, with a C-terminal 6-His tag
• Accession #: O43155
• N-terminal Sequence: Cys36
• Protein/Peptide Type: Recombinant Proteins
• Gene: FLRT2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FLRT2 Protein, CF

• Fibronectin Leucine Rich Transmembrane Protein 2
• Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2
• FLRT2
• KIAA0405
• Leucine-Rich Repeat Transmembrane Protein FLRT2

Background

FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) human FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 506 aa extracellular domain (ECD), a 21 aa TM segment and a 98 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT2 ECD shares 97%, 96%, 99%, 96% and 95% aa sequence identity with mouse, rat, equine, canine and bovine FLRT2 ECD, respectively. Human FLRT1 and FLRT3 ECDs share approximately 47% aa identity with FLRT2. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2).

1. Lacy, S. E. et al. (1999) Genomics 62:417.
2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.
3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.
4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
5. Ogata, S. et al. (2007) Genes Dev. 21:1817.

Publications for FLRT2 (2877-FL)(1)

Publication using 2877-FL

• Chiara Camillo, Nicola Facchinello, Giulia Villari, Giulia Mana, Noemi Gioelli, Chiara Sandri et al. LPHN2 inhibits vascular permeability by differential control of endothelial cell adhesion Journal of Cell Biology 2021-11-01 [PMID: 34581723] (Bioassay, Human, Primate - Chlorocebus aethiops (African Green Monkey))

Bioinformatics

• Gene Symbol: FLRT2
• Uniprot:
  • O43155()