Recombinant Human Fibulin 3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Fibulin 3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of RT4‑D6P2T rat schwannoma cells. The ED₅₀ for this effect is 0.4-2 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Fibulin 3 protein
  Gln18-Phe493 with N-terminal HA tag
• Accession #: Q12805
• N-terminal Sequence: Tyr (of HA tag)
• Protein/Peptide Type: Recombinant Proteins
• Gene: EFEMP1
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52-71 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibulin 3 Protein, CF

• DHRD
• EFEMP1
• EGF containing fibulin-like extracellular matrix protein 1
• EGF-containing fibulin-like extracellular matrix protein 1
• Extracellular protein S1-5
• FBLN3
• FBLN3DRAD
• FBNLFLJ35535
• FIBL-3
• Fibrillin-like protein
• fibrillin-like
• Fibulin 3
• fibulin-3
• MGC111353
• MLVT
• MTLV
• S1-5

Background

Human Fibulin-3, also called EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP-1), is an approximately 57 kDa member of the fibulin family of proteins (1). It was originally identified in fibroblasts from a Werner syndrome patient with premature aging (2). Along with Fibulin-4 and -5, Fibulin-3 is referred to as a class II, or short fibulin (3). Fibulin-3 contains five calcium-binding EGF-like motifs and a fibulin-type C terminus (1). Human Fibulin-3 shares 95% and 93% amino acid sequence identity with mouse and rat Fibulin-3, respectively. It has been shown to be susceptible to MMP-mediated proteolysis (4). Fibulin-3 appears to be involved in the proper function of elastic tissues. Knockout mice are viable, but display disrupted elastic fibers in fascia connective tissue of the body wall (5, 6). In humans, Fibulin-3 mutations have been associated with Doyne honeycomb retinal dystrophy (7). Its expression is up-regulated in late, metastatic stages of carcinomas, suggesting that it may play a role in cancer (8, 9). Fibulin-3 promotes the growth of gliomas and may have a pro-angiogenic role in this cancer type (10, 11). In contrast, Fibulin-3 has been shown to negatively modulate the invasiveness of lung cancer cells and inhibit self-renewal of lung cancer stem cells (12-14).

1. Giltay, R. et al. (1999) Matrix Biol. 18:469.
2. Lecka-Czernik, B. et al. (1995) Mol. Cell. Biol. 15:120.
3. Yanagisawa, H. and E.C. Davis (2010) Int. J. Biochem. Cell Biol. 42:1084.
4. Djokic, J. et al. (2013) J. Biol. Chem. 288:22821.
5. Kobayashi, N. et al. (2007) J. Biol. Chem. 282:11805.
6. McLaughlin, P.J. et al. (2007) Hum. Mol. Genet. 16:3059.
7. Stone, E.M. et al. (1999) Nat. Genet. 22:199.
8. Hwang, C.F. et al. (2010) J. Pathol. 222:367.
9. En-lin, S. et al. (2010) Gynecol. Oncol. 117:417.
10. Hu, B. et al. (2012) Cancer Res. 72:3873.
11. Nandhu, M.S. et al. (2014) Cancer Res. [Epub ahead of print].
12. Xu, S. et al. (2014) Oncol. Rep. 31:79.
13. Chen, X. et al. (2014) Carcinogenesis 35:1707.
14. Kim, I.G. et al. (2014) Oncogene 33:3908.

Publications for Fibulin-3/EFEMP1 (8416-FB)(2)

Publications using 8416-FB

• Duan, L;Tadi, MJ;O'Hara, KM;Maki, CG; Novel markers of MCL1 inhibitor sensitivity in triple negative breast cancer cells The Journal of biological chemistry 2024-05-16 [PMID: 38762181] (Bioassay, Human)
• TTD Luong, N Schelski, B Boehme, M Makridakis, A Vlahou, F Lang, B Pieske, I Alesutan, J Voelkl Fibulin-3 Attenuates Phosphate-Induced Vascular Smooth Muscle Cell Calcification by Inhibition of Oxidative Stress Cell. Physiol. Biochem., 2018-04-18;46(4):1305-1316. 2018-04-18 [PMID: 29689558] (Bioassay, Human)

Bioinformatics

• Gene Symbol: EFEMP1
• Uniprot:
  • Q12805(Human)