Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF

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1 μg/lane of Recombinant Human FGF R1 beta (IIIc) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 90 kDa and 180 kDa, respectively.
Recombinant Human FGF R1 beta (IIIc) (Catalog # 661-FR) inhibits FGF acidic-dependent cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 1-3 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1‑3 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human FGF R1 beta protein
Human FGF R1 beta (IIIc)
(Arg22-Glu285)
Accession # NP_075594
IEGRDMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Arg22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FGFR1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
56 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-95 kDa, reducing conditions
Publications
Read Publications using
661-FR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGFR1 beta (IIIc) Fc Chimera Protein, CF

  • FGF R1b
  • FGFR1 beta

Background

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1-4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1-3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform. Only the alpha  isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

  1. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
  2. Burke, D. et al. (1998) Trends Biochem. Sci. 23:59.

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Publications for FGFR1 beta (661-FR)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Binding Assay, Bioassay.


Filter By Application
Binding Assay
(1)
Bioassay
(8)
All Applications
Filter By Species
Human
(3)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 661-FR Applications Species
KK Rasmussen, MH Falkesgaar, M Winther, NK Roed, CL Quistgaard, MN Teisen, SM Edslev, DL Petersen, A Aljubouri, C Christense, PW Thulstrup, L Lo Leggio, K Teilum, PS Walmod NCAM2 Fibronectin type-III domains form a rigid structure that binds and activates the Fibroblast Growth Factor Receptor Sci Rep, 2018;8(1):8957. 2018 [PMID: 29895898] (Bioassay) Bioassay
SY Shi, YW Lu, Z Liu, J Stevens, CM Murawsky, V Wilson, Z Hu, WG Richards, ML Michaels, J Zhang, W Yan, Y Li A biparatopic agonistic antibody that mimics fibroblast growth factor 21 ligand activity J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29483191] (Bioassay) Bioassay
L Yang, M Hansen Fal, PW Thulstrup, PS Walmod, L Lo Leggio, K Krighaar R Expression, refolding and spectroscopic characterization of fibronectin type III (FnIII)-homology domains derived from human fibronectin leucine rich transmembrane protein (FLRT)-1, -2, and -3 PeerJ, 2017;5(0):e3550. 2017 [PMID: 28698826] (Bioassay, Human) Bioassay Human
Lelliott C, Ahnmark A, Admyre T, Ahlstedt I, Irving L, Keyes F, Patterson L, Mumphrey M, Bjursell M, Gorman T, Bohlooly-Y M, Buchanan A, Harrison P, Vaughan T, Berthoud H, Linden D Monoclonal antibody targeting of fibroblast growth factor receptor 1c ameliorates obesity and glucose intolerance via central mechanisms. PLoS ONE, 2014;9(11):e112109. 2014 [PMID: 25427253] (Binding Assay, Human) Binding Assay Human
Tao J, Xiang JJ, Li D, Deng N, Wang H, Gong YP Selection and characterization of a human neutralizing antibody to human fibroblast growth factor-2. Biochem. Biophys. Res. Commun., 2010;394(3):767-73. 2010 [PMID: 20227391] (Bioassay, Human) Bioassay Human
Nakayama T, Mutsuga N, Tosato G FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc. Blood, 2006;109(4):1363-72. 2006 [PMID: 17077327] (Bioassay, Mouse) Bioassay Mouse
Murase S, McKay RD A specific survival response in dopamine neurons at most risk in Parkinson&#039;s disease. J. Neurosci., 2006;26(38):9750-60. 2006 [PMID: 16988046] (Bioassay, Rat) Bioassay Rat
Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis. Development, 2005;132(6):1223-34. 2005 [PMID: 15716343] (Bioassay, Mouse) Bioassay Mouse
Kessaris N, Jamen F, Rubin LL, Richardson WD Cooperation between sonic hedgehog and fibroblast growth factor/MAPK signalling pathways in neocortical precursors. Development, 2004;131(6):1289-98. 2004 [PMID: 14960493] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol FGFR1
Uniprot