Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED₅₀ for this effect is 0.015-0.15 ng/mL in the presence of 10 µg/mL of heparin.
• Source: E. coli-derived human FGF acidic/FGF1 protein
  Phe16-Asp155, with an N-terminal Met
• Accession #: NP_000791
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: FGF1
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF acidic/FGF1 (aa 16-155) Protein

• aFGF
• alpha
• alpha-ECGF
• beta-ECGF
• ECGF
• ECGFB
• ECGF-betaAcidic fibroblast growth factor
• endothelial cell growth factor, beta
• FGF acidic
• FGF-1
• FGFABeta-endothelial cell growth factor
• FGF-alpha
• fibroblast growth factor 1 (acidic)
• GLIO703
• HBGF1
• HBGF-1
• heparin-binding growth factor 1

Background

FGF acidic, also known as FGF1, ECGF, and HBGF-1, is a 17 kDa nonglycosylated member of the FGF family of mitogenic peptides. FGF acidic, which is produced by multiple cell types, stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis (1-3). Human FGF acidic shares 54% amino acid sequence identity with FGF basic and 17%‑33% with other human FGFs. It shares 92%, 96%, 96%, and 96% aa sequence identity with bovine, mouse, porcine, and rat FGF acidic, respectively, and exhibits considerable species crossreactivity. Alternate splicing generates a truncated isoform of human FGF acidic that consists of the N-terminal 40% of the molecule and functions as a receptor antagonist (4). During its nonclassical secretion, FGF acidic associates with S100A13, copper ions, and the C2A domain of synaptotagmin 1 (5). It is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate (6). The ability of heparan sulfate to bind FGF acidic is determined by its pattern of sulfation, and alterations in this pattern during embryogenesis thereby regulate FGF acidic bioactivity (7). The association of FGF acidic with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors (8, 9). Ligation triggers receptor dimerization, transphosphorylation, and internalization of receptor/FGF complexes (10). Internalized FGF acidic can translocate to the cytosol with the assistance of Hsp90 and then migrate to the nucleus by means of its two nuclear localization signals (11-13). The phosphorylation of FGF acidic by nuclear PKC delta triggers its active export to the cytosol where it is dephosphorylated and degraded (14, 15). Intracellular FGF acidic functions as a survival factor by inhibiting p53 activity and proapoptotic signaling (16).

1. Jaye, M. et al. (1986) Science 233:541.
2. Galzie, Z. et al. (1997) Biochem. Cell Biol. 75:669.
3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
4. Yu, Y.L. et al. (1992) J. Exp. Med. 175:1073.
5. Rajalingam, D. et al. (2007) Biochemistry 46:9225.
6. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
7. Allen, B.L. and A.C. Rapraeger (2003) J. Cell Biol. 163:637.
8. Robinson, C.J. et al. (2005) J. Biol. Chem. 280:42274.
9. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
10. Wiedlocha, A. and V. Sorensen (2004) Curr. Top. Microbiol. Immunol. 286:45.
11. Wesche, J. et al. (2006) J. Biol. Chem. 281:11405.
12. Imamura, T. et al. (1990) Science 249:1567.
13. Wesche, J. et al. (2005) Biochemistry 44:6071.
14. Wiedlocha, A. et al. (2005) Mol. Biol. Cell 16:794.
15. Nilsen, T. et al. (2007) J. Biol. Chem. 282:26245.
16. Bouleau, S. et al. (2005) Oncogene 24:7839.

Publications for FGF acidic/FGF1 (232-FA)(23)

Publications using 232-FA

• Mokhtaridoost, M;Chalmers, JJ;Soleimanpoor, M;McMurray, BJ;Lato, DF;Nguyen, SC;Musienko, V;Nash, JO;Espeso-Gil, S;Ahmed, S;Delfosse, K;Browning, JWL;Barutcu, AR;Wilson, MD;Liehr, T;Shlien, A;Aref, S;Joyce, EF;Weise, A;Maass, PG; Inter-chromosomal contacts demarcate genome topology along a spatial gradient Nature communications 2024-11-13 [PMID: 39532865] (Bioassay, Human)
• Ding, K;Chen, L;Levine, K;Sikora, M;Tasdemir, N;Dabbs, D;Jankowitz, R;Hazan, R;Shah, OS;Atkinson, JM;Lee, AV;Oesterreich, S; Estrogen regulation and functional role of FGFR4 in estrogen receptor positive breast cancer bioRxiv : the preprint server for biology 2024-03-20 [PMID: 38562741] (Bioassay, Human)
• Huang, HW;Yang, CM;Yang, CH; Beneficial Effects of Fibroblast Growth Factor-1 on Retinal Pigment Epithelial Cells Exposed to High Glucose-Induced Damage: Alleviation of Oxidative Stress, Endoplasmic Reticulum Stress, and Enhancement of Autophagy International journal of molecular sciences 2024-03-11 [PMID: 38542166] (Bioassay, Human)
• Y Shinmyo, K Saito, T Hamabe-Hor, N Kameya, A Ando, K Kawasaki, TAD Duong, M Sakashita, J Roboon, T Hattori, T Kannon, K Hosomichi, M Slezak, MG Holt, A Tajima, O Hori, H Kawasaki Localized astrogenesis regulates gyrification of the cerebral cortex Science Advances, 2022-03-11;8(10):eabi5209. 2022-03-11 [PMID: 35275722] (Bioassay, Mouse)
• HW Huang, CM Yang, CH Yang Fibroblast Growth Factor Type 1 Ameliorates High-Glucose-Induced Oxidative Stress and Neuroinflammation in Retinal Pigment Epithelial Cells and a Streptozotocin-Induced Diabetic Rat Model International Journal of Molecular Sciences, 2021-07-05;22(13):. 2021-07-05 [PMID: 34281287] (Bioassay, Human)
• Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi FGF signal is not required for hepatoblast differentiation of human iPS cells Sci Rep, 2019-03-06;9(1):3713. 2019-03-06 [PMID: 30842525] (Bioassay, Human)
• A Taguchi, R Nishinakam Higher-Order Kidney Organogenesis from Pluripotent Stem Cells Cell Stem Cell, 2017-11-09;0(0):. 2017-11-09 [PMID: 29129523] (Bioassay, Human)
• Ilkow C, Marguerie M, Batenchuk C, Mayer J, Ben Neriah D, Cousineau S, Falls T, Jennings V, Boileau M, Bellamy D, Bastin D, de Souza C, Alkayyal A, Zhang J, Le Boeuf F, Arulanandam R, Stubbert L, Sampath P, Thorne S, Paramanthan P, Chatterjee A, Strieter R, Burdick M, Addison C, Stojdl D, Atkins H, Auer R, Diallo J, Lichty B, Bell J Reciprocal cellular cross-talk within the tumor microenvironment promotes oncolytic virus activity. Nat Med, 2015-04-20;21(5):530-6. 2015-04-20 [PMID: 25894825] (Bioassay, Human)
• Tucker , Budd A, Mullins , Robert F, Streb , Luan M, Anfinson , Kristin, Eyestone , Mari E, Kaalberg , Emily, Riker , Megan J, Drack , Arlene V, Braun , Terry A, Stone , Edwin M Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa. Elife, 2013-08-27;2(0):e00824. 2013-08-27 [PMID: 23991284] (Bioassay, Human)
• Tucker B, Anfinson K, Mullins R, Stone E, Young M Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation. Stem Cells Transl Med, 2012-12-27;2(1):16-24. 2012-12-27 [PMID: 23283489] (Bioassay, Human)
• Luo X, Hutley LJ, Webster JA, Kim YH, Liu DF, Newell FS, Widberg CH, Bachmann A, Turner N, Schmitz-Peiffer C, Prins JB, Yang GS, Whitehead JP Identification of BMP and activin membrane-bound inhibitor (BAMBI) as a potent negative regulator of adipogenesis and modulator of autocrine/paracrine adipogenic factors. Diabetes, 2012-01-01;61(1):124-36. 2012-01-01 [PMID: 22187378] (Bioassay, Human)
• Roelandt P, Pauwelyn KA, Sancho-Bru P Human embryonic and rat adult stem cells with primitive endoderm-like phenotype can be fated to definitive endoderm, and finally hepatocyte-like cells. PLoS ONE, 2010-08-11;5(8):e12101. 2010-08-11 [PMID: 20711405] (Cell Culture, Human)
• Darby S, Murphy T, Thomas H, Robson CN, Leung HY, Mathers ME, Gnanapragasam VJ Similar expression to FGF (Sef) inhibits fibroblast growth factor-induced tumourigenic behaviour in prostate cancer cells and is downregulated in aggressive clinical disease. Br. J. Cancer, 2009-11-03;101(11):1891-9. 2009-11-03 [PMID: 19888221] (Bioassay, Human)
• Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice. Stem Cells, 2008-05-15;26(7):1778-86. 2008-05-15 [PMID: 18483422] (Bioassay, Mouse)
• Patel VN, Likar KM, Zisman-Rozen S, Cowherd SN, Lassiter KS, Sher I, Yates EA, Turnbull JE, Ron D, Hoffman MP Specific heparan sulfate structures modulate FGF10-mediated submandibular gland epithelial morphogenesis and differentiation. J. Biol. Chem., 2008-01-28;283(14):9308-17. 2008-01-28 [PMID: 18230614] (Bioassay, Mouse)
• Patel VN, Knox SM, Likar KM, Lathrop CA, Hossain R, Eftekhari S, Whitelock JM, Elkin M, Vlodavsky I, Hoffman MP Heparanase cleavage of perlecan heparan sulfate modulates FGF10 activity during ex vivo submandibular gland branching morphogenesis. Development, 2007-10-24;134(23):4177-86. 2007-10-24 [PMID: 17959718] (Bioassay, Mouse)
• Lin BC, Wang M, Blackmore C, Desnoyers LR Liver-specific activities of FGF19 require Klotho beta. J. Biol. Chem., 2007-07-11;282(37):27277-84. 2007-07-11 [PMID: 17627937] (Bioassay, Human)
• Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro. Int. J. Dev. Biol., 2006-01-01;50(7):645-52. 2006-01-01 [PMID: 16892178] (Bioassay, Human)
• Masih-Khan E, Trudel S, Heise C, Li Z, Paterson J, Nadeem V, Wei E, Roodman D, Claudio JO, Bergsagel PL, Stewart AK MIP-1alpha (CCL3) is a downstream target of FGFR3 and RAS-MAPK signaling in multiple myeloma. Blood, 2006-07-18;108(10):3465-71. 2006-07-18 [PMID: 16849642] (Bioassay, Human)
• Shin JW, Min M, Larrieu-Lahargue F, Canron X, Kunstfeld R, Nguyen L, Henderson JE, Bikfalvi A, Detmar M, Hong YK Prox1 promotes lineage-specific expression of fibroblast growth factor (FGF) receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis. Mol. Biol. Cell, 2005-11-16;17(2):576-84. 2005-11-16 [PMID: 16291864] (Bioassay, Human)
• Steinberg Z, Myers C, Heim VM, Lathrop CA, Rebustini IT, Stewart JS, Larsen M, Hoffman MP FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis. Development, 2005-02-16;132(6):1223-34. 2005-02-16 [PMID: 15716343] (Bioassay, Mouse)
• Ohashi K, Waugh JM, Dake MD, Yokoyama T, Kuge H, Nakajima Y, Yamanouchi M, Naka H, Yoshioka A, Kay MA Liver tissue engineering at extrahepatic sites in mice as a potential new therapy for genetic liver diseases. Hepatology, 2005-01-01;41(1):132-40. 2005-01-01 [PMID: 15619229] (In Vivo, Mouse)
• Dame MK, Yu X, Garrido R, Bobrowski W, McDuffie JE, Murphy HS, Albassam M, Varani J A stepwise method for the isolation of endothelial cells and smooth muscle cells from individual canine coronary arteries. In Vitro Cell. Dev. Biol. Anim., 2003-11-01;39(10):402-6. 2003-11-01 [PMID: 14690451] (Bioassay, Canine)

Bioinformatics

• Gene Symbol: FGF1
• Uniprot:
  • NP_000791()