Recombinant Human Fas Ligand/TNFSF6 Protein, CF


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Recombinant Human Fas Ligand/TNFSF6 Protein, CF Summary

Details of Functionality
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.3‑1.5 ng/mL in the presence of 10 µg/mL of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Chinese Hamster Ovary cell line, CHO-derived human Fas Ligand/TNFSF6 protein
Pro134-Leu281, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19 - 32 kDa, reducing conditions
Read Publications using
126-FL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fas Ligand/TNFSF6 Protein, CF

  • apoptosis (APO-1) antigen ligand 1
  • Apoptosis antigen ligand
  • APT1LG1CD95L
  • APTL
  • CD178 antigen
  • CD178
  • CD95L
  • CD95-L
  • Fas antigen ligand
  • Fas ligand (TNF superfamily, member 6)
  • Fas Ligand
  • FASLCD95 ligand
  • TNFSF6
  • TNFSF6FasL
  • tumor necrosis factor (ligand) superfamily, member 6
  • tumor necrosis factor ligand superfamily member 6


Fas Ligand (FasL), also known as CD178, CD95L, or TNFSF6, is a 40 kDa type II transmembrane member of the TNF superfamily of proteins. Its ability to induce apoptosis in target cells plays an important role in the development, homeostasis, and function of the immune system (1). Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain (2). Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Both mouse and human Fas Ligand are active on mouse and human cells (2, 3). Fas Ligand is expressed on the cell surface as a nondisulfide-linked homotrimer on activated CD4+ Th1 cells, CD8+ cytotoxic T cells, and NK cells (1). Fas Ligand binding to Fas/CD95 on an adjacent cell triggers apoptosis in the Fas‑expressing cell (2, 4). Fas Ligand also binds DcR3 which is a soluble decoy receptor that interferes with Fas Ligand-induced apoptosis (5). Fas Ligand can be released from the cell surface by metalloproteinases as a 26 kDa soluble molecule which remains trimeric (6, 7). Shed Fas Ligand retains the ability to bind Fas, although its ability to trigger apoptosis is dramatically reduced (6, 7). In the absence of TGF‑ beta , however, Fas Ligand/Fas interactions instead promote neutrophil-mediated inflammatory responses (3, 8). Fas Ligand itself transmits reverse signals that costimulate the proliferation of freshly antigen-stimulated T cells (9). Fas Ligand-induced apoptosis plays a central role in the development of immune tolerance and the maintance of immune privileged sites (10). This function is exploited by tumor cells which evade immune surveillance by upregulating Fas Ligand to kill tumor infiltrating lymphocytes (8, 11). In gld mice, a Fas Ligand point mutation is the cause of severe lymphoproliferation and systemic autoimmunity (12, 13).
  1. Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
  2. Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
  3. Seino, K-I. et al. (1998) J. Immunol. 161:4484.
  4. Suda, T. et al. (1993) Cell 75:1169.
  5. Pitti, R.M. et al. (1998) Nature 396:699.
  6. Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
  7. Tanaka, M. et al. (1998) Nature Med. 4:31.
  8. Chen, J.-J. et al. (1998) Science 282:1714.
  9. Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. 97:1707.
  10. Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
  11. Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
  12. Takahashi, T. et al. (1994) Cell 76:969.
  13. Lynch, D.H. et al. (1994) Immunity 1:131.

Publications for Fas Ligand/TNFSF6 (126-FL/CF)(20)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, Complex Species Category, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 3 applications: Bioassay, Surface Plasmon Resonance, Surface Plasmon Resonance (SPR.

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Surface Plasmon Resonance
Surface Plasmon Resonance (SPR
All Applications
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Complex Species Category
Primate - Macaca mulatta (Rhesus Macaque)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 126-FL/CF Applications Species
W Zhu, H Chu, Y Zhang, T Luo, H Yu, H Zhu, Y Liu, H Gao, Y Zhao, Q Li, X Wang, G Li, W Yang Fructose-1,6-bisphosphatase 1 dephosphorylates IkappaBalpha and suppresses colorectal tumorigenesis Cell Research, 2023-01-16;0(0):. 2023-01-16 [PMID: 36646759] (Bioassay, Human) Bioassay Human
Bhuyan S, Pal B, Pathak L, Saikia P, Mitra S, Gayan S, Mokhtari R, Li H, Ramana C, Baishya D, Das B Targeting hypoxia-induced tumor stemness by activating pathogen-induced stem cell niche defense. Front Immunol, 2022-09-29;13(0):933329. 2022-09-29 [PMID: 36248858] (Bioassay, Complex Species Category) Bioassay Complex Species Category
F Velard, A Chatron-Co, D Côme, MD Ah-Kioon, H Lin, N Hafsia, M Cohen-Sola, HK Ea, F Lioté Adrenomedullin and truncated peptide adrenomedullin(22-52) affect chondrocyte response to apoptotis in vitro: downregulation of FAS protects chondrocyte from cell death Sci Rep, 2020-10-07;10(1):16740. 2020-10-07 [PMID: 33028903] (Bioassay, Bovine) Bioassay Bovine
AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 2018-03-05;0(0):1-43. 2018-03-05 [PMID: 29436901] (Surface Plasmon Resonance (SPR, Human) Surface Plasmon Resonance (SPR Human
M Frank-Bert, DS Pisetsky, C Kolling, BA Michel, RE Gay, A Jüngel, S Gay TLR3 Ligand Poly(I:C) Exerts Distinct Actions in Synovial Fibroblasts When Delivered by Extracellular Vesicles Front Immunol, 2018-01-29;9(0):28. 2018-01-29 [PMID: 29434584] (Bioassay, Human) Bioassay Human
N Han, L Shi, Q Guo, W Sun, Y Yu, L Yang, X Zhang, M Zhang HAT1 induces lung cancer cell apoptosis via up regulating Fas Oncotarget, 2017-09-23;8(52):89970-89977. 2017-09-23 [PMID: 29163803] (Bioassay, Human) Bioassay Human
Bo Ra You, Bo Ram Han, Woo Hyun Park Suberoylanilide hydroxamic acid increases anti-cancer effect of tumor necrosis factor-? through up-regulation of TNF receptor 1 in lung cancer cells. Oncotarget, 2017-03-14;0(0):1949-2553. 2017-03-14 [PMID: 28099148] (Bioassay, Human) Bioassay Human
Endotoxemia contributes to CD27+ memory B-cell apoptosis via enhanced sensitivity to Fas ligation in patients with Cirrhosis Sci Rep, 2016-11-18;6(0):36862. 2016-11-18 [PMID: 27857173] (Bioassay, Human) Bioassay Human
Cui X, Zhang L, Magli A, Catera R, Yan X, Griffin D, Rothstein T, Barrientos J, Kolitz J, Allen S, Rai K, Chiorazzi N, Chu C Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability. Leukemia, 2015-07-29;30(1):74-85. 2015-07-29 [PMID: 26220042] (Bioassay, Human) Bioassay Human
Liang D, Hou Y, Lou X, Chen H Decoy Receptor 3 Improves Survival in Experimental Sepsis by Suppressing the Inflammatory Response and Lymphocyte Apoptosis. PLoS ONE, 2015-06-29;10(6):e0131680. 2015-06-29 [PMID: 26121476] (Bioassay, Human) Bioassay Human
Show All 20 Publications.

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Gene Symbol FASLG