Recombinant Human Ephrin-B1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Ephrin-B1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse EphB3 Fc Chimera (Catalog # 432-B3) is coated at 2 μg/mL, Recombinant Human Ephrin-B1 Fc Chimera binds with an apparent K_D <0.2 nM.
• Source: Mouse myeloma cell line, NS0-derived human Ephrin-B1 protein
  

  Human Ephrin-B1 (Leu28-Ser236) Accession # P98172	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P98172
• N-terminal Sequence: Leu28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: EFNB1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-67 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ephrin-B1 Fc Chimera Protein, CF

• Cek5-L
• EFL3
• EFL-3
• EFNB1
• ELK ligand
• ELK-L
• EphrinB1
• Ephrin-B1
• LERK-2
• STRA-1

Background

Ephrin-B1, also known as Elk Ligand, LERK2, and Eplg2, is an approximately 45 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B1 preferentially interacts with receptors in the EphB family. The binding of Ephrin-B1 to EphB proteins also triggers reverse signaling through Ephrin-B1 (1, 2). Mature human Ephrin-B1 consists of a 210 amino acid (aa) ECD, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain (3, 4). Within the ECD, human Ephrin-B1 shares approximately 94% aa sequence identity with mouse and rat Ephrin-B1. Ligation by EphB2 enhances shedding of a 35 kDa fragment of the Ephrin-B1 ECD (5). The residual membrane-bound portion is then cleaved by gamma-secretase to release the intracellular domain (6). Ephrin-B1 also associates in cis with Claudin-1, -4, and -5 (7, 8). It is expressed on glomerular podocyte slit diaphragms, developing thymocytes, peripheral T cells, monocytes, macrophages, vascular endothelial cells, cardiomyocytes, osteoclasts, and luteinizing granulosa cells in the ovary (8-13). In the developing nervous system, Ephrin-B1 plays a role in cellular migration, axon guidance, and presynaptic development (14-16). It also regulates developing thymocyte survival, monocyte migration, osteoclast differentiation and function, cardiac muscle morphogenesis, and tumorigenesis (5, 8, 10-12). Ephrin-B1 is up‑regulated on reactive astrocytes and on macrophages and T cells found in atherosclerotic plaques (11, 17).

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
3. Davis, S. et al. (1994) Science 266:816.
4. Beckmann, M.P. et al. (1994) EMBO J. 13:3757.
5. Tanaka, M. et al. (2007) J. Cell Sci. 120:2179.
6. Tomita, T. et al. (2006) Mol. Neurodegen. 1:2.
7. Tanaka, M. et al. (2005) EMBO J. 24:3700.
8. Genet, G. et al. (2012) Circ. Res. 110:688.
9. Hashimoto, T. et al. (2007) Kidney Int. 72:954.
10. Yu, G. et al. (2006) J. Biol. Chem. 281:10222.
11. Sakamoto, A. et al. (2008) Clin. Sci. 114:643.
12. Cheng, S. et al. (2012) PLoS ONE 7:e32887.
13. Egawa, M. et al. (2003) J. Clin. Endocrinol. Metab. 88:4384.
14. Davy, A. et al. (2004) Genes Dev. 18:572.
15. Bush, J.O. and P. Soriano (2009) Genes Dev. 23:1586.
16. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20487.
17. Wang, Y. et al. (2005) Eur. J. Neurosci. 21:2336.

Publications for Ephrin-B1 (7654-EB)(5)

Publications using 7654-EB

• D Vreeken, CS Bruikman, SML Cox, H Zhang, R Lalai, A Koudijs, AJ van Zonnev, GK Hovingh, JM van Gils EPH receptor B2 stimulates human monocyte adhesion and migration independently of its EphrinB ligands J. Leukoc. Biol., 2020-04-26;0(0):. 2020-04-26 [PMID: 32337793] (Cell Culture, Human)
• MJ Wagner, MS Hsiung, GD Gish, RD Bagshaw, SA Doodnauth, MA Soliman, C Jørgensen, M Tucholska, R Rottapel The Shb scaffold binds the Nck adaptor protein, p120 RasGAP and Chimaerins and thereby facilitates heterotypic cell segregation by the receptor EphB2 J. Biol. Chem., 2020-02-14;0(0):. 2020-02-14 [PMID: 32060095] (Bioassay, Human)
• F Cutrale, D Rodriguez, V Hortigüela, CL Chiu, J Otterstrom, S Mieruszyns, A Seriola, E Larrañaga, A Raya, M Lakadamyal, SE Fraser, E Martinez, S Ojosnegros Using enhanced number and brightness to measure protein oligomerization dynamics in live cells Nat Protoc, 2019-02-01;14(2):616-638. 2019-02-01 [PMID: 30675035] (Bioassay, Human)
• A Arthur, TM Nguyen, S Paton, A Klisuric, ACW Zannettino, S Gronthos The osteoprogenitor-specific loss of ephrinB1 results in an osteoporotic phenotype affecting the balance between bone formation and resorption Sci Rep, 2018-08-24;8(1):12756. 2018-08-24 [PMID: 30143786] (Bioassay, Human)
• Can Gao Blocking the Interaction between EphB2 and ADDLs by a Small Peptide Rescues Impaired Synaptic Plasticity and Memory Deficits in a Mouse Model of Alzheimer&#039;s Disease J. Neurosci., 2016-11-23;36(47):11959-11973. 2016-11-23 [PMID: 27881781] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: EFNB1
• Uniprot:
  • P98172()