Recombinant Human EDA-A2 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human EDA-A2 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human EDA2R/TNFRSF27/XEDAR Fc Chimera (Catalog # 1093-XD) is coated at 0.1  μg/mL (100 μL/well), the concentration of Recombinant Human EDA-A2/Ectodysplasin A2 that produces 50% of the optimal binding response is 3-15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human EDA-A2/Ectodysplasin A2 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human EDA-A2
(Ala179-Ser389)
Accession # AAC77371
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
EDA
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
24.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
28-38 kDa, reducing conditions
Publications
Read Publication using
922-ED in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EDA-A2 Protein

  • EDAA2
  • EDA-A2

Background

EDA-A2, a product of the EDA gene (also called Tabby), is a type II transmembrane protein that is a member of the TNF Superfamily (TNFSF). Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. The extracellular domains of human and mouse EDA-A2 share approximately 94% identity. Within the TNFSF, EDA-A2 shares the highest homology with EDA-A1, the other product of the EDA gene. EDA-A2 and EDA-A1 are splice variants of EDA which differ by only two amino acids. EDA-A1 contains two additional amino acids, Glu 308 and Val 309. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor Superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF family member with high homology to EDAR. Mutations in EDA-A1, EDA-A2, EDAR and XEDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene.

  1. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
  2. Kere, J. et al. (1996) Nat. Genet. 13:409.
  3. Monreal, A.W. et al. (1998) Am. J. Hum. Genet. 63:380.
  4. Headon, D.J. and P.A. Overbeek (1999) Nat. Genet. 22(4):370.
  5. Kumar, A. et al. (2000) J. Biol. Chem. 276(4):2668.
  6. Monreal, A.W. et al. (1999) Nat. Genet. 22(4):366.
  7. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94(24):13069.
  8. Yan, M. et al. (2000) Science 290(5491):523.

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Publications for EDA-A2/Ectodysplasin A2 (922-ED)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol EDA
Entrez
Uniprot