Recombinant Human DLL4 Fc Chimera Protein, CF

Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) has a molecular weight (MW) of 168-185 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Equivalent binding activity of GMP (BT-DLL4-GMP) and RUO (Catalog # BT-DLL4) grades of RecombinantHuman DLL4 as measured in a functional ELISA with Recombinant Human Notch-1 Protein (orange and red, respectively).

In a functional ELISA, Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) binds to Recombinant Human Notch-1 Fc Chimera (3647-TK) with an ED50 of 2.00-20.0 ng/mL.

2 μg/lane of Recombinant Human DLL4 Fc Chimera Protein (Catalog # BT-DLL4) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 89-98 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human DLL4 Fc Chimera Protein, CF Summary

• Additional Information: Analyzed by SEC-MALS
• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human DLL4 Fc Chimera (Catalog # BT-DLL4) binds to Recombinant Human Notch-1 Fc Chimera (Catalog # 3647-TK) with an ED₅₀ of 2.00-20.0 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human DLL4 protein
  

  Human DLL4 (Ser27-Pro524) Accession # Q9NR61.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• N-terminal Sequence: Ser 27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 89-98 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HBS and Tween®-80 with Trehalose.
• Purity: >95%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions:

  Reconstitute the 20 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DLL4 Fc Chimera Protein, CF

• Delta 4 precursor
• delta 4
• delta ligand 4
• delta4
• delta-like 4 (Drosophila)
• delta-like 4 homolog (Drosophila)
• delta-like 4 homolog
• delta-like 4 protein
• delta-like protein 4
• DLL4
• Drosophila Delta homolog 4
• hdelta2
• MGC126344
• notch ligand delta-2
• notch ligand DLL4

Background

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal regulated intramembrane proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by a disintegrin metalloprotease (ADAM) and then by presenilin/ gamma secretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1). The use of DLL4, in conjunction with VCAM1, has been shown as an effective method for generating hematopoietic progenitors and T cells from hPSCs in a serum- and feeder-free environment.

1. Shutter, J.R. et al. (2000) Genes Dev. 14:1313.
2. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543.
3. Walker, L. et al. (2001) Stem Cells 19:543.
4. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113.
5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
6. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.