Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL, 100 μL/well, will mediate >25% BALB/3T3 cell adhesion
  (3 x 10⁴ cell/well added).
• Source: Chinese Hamster Ovary cell line, CHO-derived human Cyr61/CCN1 protein
  

  Human Cyr61 (Ala22-Asp381) Accession # O00622	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: O00622
• N-terminal Sequence: Ala22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CYR61
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Cyr61/CCN1 Fc Chimera Protein, CF

• CCN family member 1
• CCN1
• CCN1IGF-binding protein 10
• Cyr61
• Cysteine-rich angiogenic inducer 61
• cysteine-rich, angiogenic inducer, 61
• GIG1
• GIG1cysteine-rich heparin-binding protein 61
• IBP-10
• IGFBP-10
• IGFBP10cysteine-rich, anigogenic inducer, 61
• Insulin-like growth factor-binding protein 10
• protein CYR61
• Protein GIG1

Background

Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, when
down-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
2. Jay, P. et al. (1997) Oncogene 14:1753.
3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
5. Leu, S.-J. et al. (2002) J. Biol. Chem. 277:46248.
6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
10. Xie, D. et al. (2004) Cancer Res. 64:1987.
11. Lin, M.-T. et al. (2007) J. Biol. Chem. 282:34594.
12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
16. Chen, C.-C. et al. (2001) J. Biol. Chem. 276:47329.

Publications for Cyr61/CCN1 (4055-CR)(5)

Publications using 4055-CR

• Y Liu, BL Tang, ML Lu, HX Wang Astragaloside IV improves pulmonary arterial hypertension by increasing the expression of CCN1 and activating the ERK1/2 pathway Journal of Cellular and Molecular Medicine, 2023-02-10;0(0):. 2023-02-10 [PMID: 36762748] (Bioassay, Human)
• JH Won, JS Choi, JI Jun CCN1 interacts with integrins to regulate intestinal stem cell proliferation and differentiation Nature Communications, 2022-06-03;13(1):3117. 2022-06-03 [PMID: 35660741] (Bioassay, Mouse)
• CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation Proc Natl Acad Sci USA, 2016-08-01;113(33):E4847-56. 2016-08-01 [PMID: 27482114] (Flow Cytometry, Mouse)
• Comes , Stefania, Gagliardi , Miriam, Laprano , Nicola, Fico , Annalisa, Cimmino , Amelia, Palamidessi , Andrea, De Cesare , Dario, De Falco , Sandro, Angelini , Claudia, Scita , Giorgio, Patriarca , Eduardo, Matarazzo , Maria R, Minchiotti , Gabriell L-Proline induces a mesenchymal-like invasive program in embryonic stem cells by remodeling H3K9 and H3K36 methylation. Stem Cell Reports, 2013-10-10;1(4):307-21. 2013-10-10 [PMID: 24319666] (Bioassay, Mouse)
• Craig TA, Bhattacharya R, Mukhopadhyay D, Kumar R Sclerostin binds and regulates the activity of cysteine-rich protein 61. Biochem. Biophys. Res. Commun., 2009-12-31;392(1):36-40. 2009-12-31 [PMID: 20043874] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: CYR61
• Uniprot:
  • O00622(Human)