Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein

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RecombinantHuman CXCL3/GRO gamma (Catalog # 8297-GG) chemoattracts BaF3 mouse pro B cellstransfected with human CXCR2. The ED50 for this effect is 0.4-2.4ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.4-2.4 ng/mL.
Source
E. coli-derived human CXCL3/GRO gamma/CINC-2/DCIP-1 protein
Thr39-Asn107
Accession #
N-terminal Sequence
Thr39
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL3
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CXCL3/GRO gamma (aa 39-107) Protein

  • chemokine (C-X-C motif) ligand 3
  • CINC-2
  • CINC-2b
  • C-X-C motif chemokine 3
  • CXCL3
  • Dcip1
  • Gm1960
  • GRO gamma
  • GRO3 oncogene
  • GRO3
  • GROG
  • GRO-gamma
  • GRO-gamma(1-73)
  • Growth-regulated protein gamma
  • Macrophage inflammatory protein 2-beta
  • melanoma growth stimulatory activity gamma
  • MGSA gamma
  • MIP2B
  • MIP-2b
  • MIP2-beta
  • SCYB3

Background

CXCL3, also known as Growth-regulated Oncogene gamma (GRO gamma), Macrophage Inflammatory Protein 2 beta (MIP-2beta), Dendritic Cell Inflammatory Protein 1 (DCIP-1), or Cytokine-induced Neutrophil Attractant 2 (CINC-2), is an 8 kDa pro-inflammatory member of the CXC subfamily of heparin-binding chemokines (1-3). Mature human CXCL3 (aa 35-107) shares 70% and 74% amino acid (aa) sequence identity with mouse and rat CXCL3, respectively. CXCL3 binds and activates CXCR2 to induce chemoattraction of CXCR2-expressing cells including neutrophils and endothelial cells (4, 5). Additional N-terminal processing of mature CXCL3 by the removal of aa 35-38 increases its chemotactic activity by several fold (6). In addition to binding CXCR2, CXCL3 can also bind and be sequestered by the Duffy Antigen Receptor for Chemokines (DARC) decoy receptor (7).

  1. Tekamp-Olson, P. et al. (1990) J. Exp. Med. 172:911.
  2. Haskill, S. et al. (1990) Proc. Natl. Acad. Sci. USA 87:7732.
  3. Zlotnik, A. and O. Yoshie (2012) Immunity 36:705.
  4. Strydom, N. and S.M. Rankin (2013) J. Innate Immun. 5:304.
  5. Mehrad, B. et al. (2007) Thromb. Haemost. 97:755.
  6. Wuyts, A. et al. (1999) Eur. J. Biochem. 260:421.
  7. Hansell, C.A. et al. (2011) Immunol. Cell Biol. 89:197.

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Bioinformatics

Gene Symbol CXCL3
Entrez
Uniprot