Recombinant Human CRTAM Fc Chimera Protein, CF

Images

 
There are currently no images for CRTAM (1695-CR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human CRTAM Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding activity in a functional ELISA. Galiberet, L. et al. (2005) J. Biol. Chem. 280:21955. Immobilized rhCRTAM/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhIGSF4A (Catalog# 3519-S4) with a linear range of 3-200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CRTAM protein
Human CRTAM
(Ser18 - Ser286)
Accession # O95727
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser18
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
CRTAM
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
56.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-95 kDa, reducing conditions
Publications
Read Publication using
1695-CR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CRTAM Fc Chimera Protein, CF

  • CD355 antigen
  • CD355
  • class I MHC restricted T cell associated molecule
  • class-I MHC-restricted T cell associated molecule
  • Class-I MHC-restricted T-cell-associated molecule
  • CRTAM
  • cytotoxic and regulatory T cell molecule
  • cytotoxic and regulatory T-cell molecule

Background

CRTAM (Class I-restricted T cell-associated molecule) is a nectin family member of the immunoglobulin superfamily that is expressed by activated CD8+ and NK T cells (1 - 4). NK activation receptor engagement, but not cytokines, also induce NK CRTAM expression (4, 5). CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum (1, 2). Human CRTAM is a 393 amino acid (aa), 80 kDa type I transmembrane glycoprotein with a 17 aa signal sequence, a 269 aa extracellular domain (ECD), a 21 aa transmembrane segment and an 84 aa cytoplasmic domain. The ECD has one V-type and one C1-type Ig-like domain, while the cytoplasmic region shows a potential class I PDZ domain (1 - 5). Human CRTAM ECD shows 70%, 43% and 63% aa identity with mouse, rat and canine CRTAM ECD, respectively, but 73 - 78% aa identity within the Ig-like domains. The V-type Ig-like domain mediates interaction with the corresponding domain on another nectin family member, IGSF4 (also called TSLC-1, Necl-2, Syncam or SgIGSF) (4, 5). CRTAM is a homodimer on the cell surface but does not show homotypic binding in trans (3 - 5). The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions (3 - 5). IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8+ cells induces increased IFN-gamma or IL-22 production (3, 4). A role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed (3 - 5). IGSF4 is expressed broadly, including on epithelia, neurons, a subset of tonsillar B cells (4, 5), and a rare splenic T zone-restricted BCDA3+ dendritic cell population which interacts with CRTAM (3).

  1. Kennedy, J. et al. (2000) J. Leukoc. Biol. 67:725.
  2. Patino-Lopez, G. et al. (2006) J. Neuroimmunol. 171:145.
  3. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
  4. Boles, K. S. et al. (2005) Blood 106:779.
  5. Arase, N. et al. (2005) Int. Immunol. 17:1227.

Customers Who Viewed This Item Also Viewed...

NB300-186
Species: Hu, Rt
Applications: WB, Simple Western, ICC/IF
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
MAB19081
Species: Hu
Applications: Flow, CyTOF-ready
NBP2-62627
Species: Hu
Applications: IHC, IHC-P
MAB7089
Species: Mu
Applications: Flow, IHC, CyTOF-ready
MAB2048
Species: Hu
Applications: WB, Flow, CyTOF-ready
AF2426
Species: Hu
Applications: WB, IHC
AF144
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
AF7286
Species: Mu
Applications: Flow, CyTOF-ready
6507-IL/CF
Species: Hu
AF3126
Species: Hu
Applications: Flow, CyTOF-ready
MAB689
Species: Hu
Applications: WB, Flow, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
AF6585
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
MAB1278
Species: Hu
Applications: Flow, AgAct, CyTOF-ready
MAB356
Species: Hu
Applications: WB, Flow, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-80894
Species: Hu
Applications: WB, IHC, IHC-P

Publications for CRTAM (1695-CR)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for CRTAM (1695-CR) (0)

There are no reviews for CRTAM (1695-CR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CRTAM (1695-CR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

IL-4
6507-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human CRTAM Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CRTAM
Uniprot