Recombinant Human CRTAM Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human CRTAM Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding activity in a functional ELISA. Galiberet, L. et al. (2005) J. Biol. Chem. 280:21955. Immobilized rhCRTAM/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhIGSF4A (Catalog# 3519-S4) with a linear range of 3-200 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human CRTAM protein
  

  Human CRTAM (Ser18 - Ser286) Accession # O95727	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: O95727
• N-terminal Sequence: Ser18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CRTAM
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 56.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CRTAM Fc Chimera Protein, CF

• CD355 antigen
• CD355
• class I MHC restricted T cell associated molecule
• class-I MHC-restricted T cell associated molecule
• Class-I MHC-restricted T-cell-associated molecule
• CRTAM
• cytotoxic and regulatory T cell molecule
• cytotoxic and regulatory T-cell molecule

Background

CRTAM (Class I-restricted T cell-associated molecule) is a nectin family member of the immunoglobulin superfamily that is expressed by activated CD8⁺ and NK T cells (1 - 4). NK activation receptor engagement, but not cytokines, also induce NK CRTAM expression (4, 5). CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum (1, 2). Human CRTAM is a 393 amino acid (aa), 80 kDa type I transmembrane glycoprotein with a 17 aa signal sequence, a 269 aa extracellular domain (ECD), a 21 aa transmembrane segment and an 84 aa cytoplasmic domain. The ECD has one V-type and one C1-type Ig-like domain, while the cytoplasmic region shows a potential class I PDZ domain (1 - 5). Human CRTAM ECD shows 70%, 43% and 63% aa identity with mouse, rat and canine CRTAM ECD, respectively, but 73 - 78% aa identity within the Ig-like domains. The V-type Ig-like domain mediates interaction with the corresponding domain on another nectin family member, IGSF4 (also called TSLC-1, Necl-2, Syncam or SgIGSF) (4, 5). CRTAM is a homodimer on the cell surface but does not show homotypic binding in trans (3 - 5). The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions (3 - 5). IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8⁺ cells induces increased IFN-gamma or IL-22 production (3, 4). A role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed (3 - 5). IGSF4 is expressed broadly, including on epithelia, neurons, a subset of tonsillar B cells (4, 5), and a rare splenic T zone-restricted BCDA3+ dendritic cell population which interacts with CRTAM (3).

1. Kennedy, J. et al. (2000) J. Leukoc. Biol. 67:725.
2. Patino-Lopez, G. et al. (2006) J. Neuroimmunol. 171:145.
3. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
4. Boles, K. S. et al. (2005) Blood 106:779.
5. Arase, N. et al. (2005) Int. Immunol. 17:1227.

Publications for CRTAM (1695-CR)(2)

Publications using 1695-CR

• Mónica Díaz-Coránguez, José Segovia, Adolfo López-Ornelas, Henry Puerta-Guardo, Juan Ludert, Bibiana Chávez et al. Transmigration of Neural Stem Cells across the Blood Brain Barrier Induced by Glioma Cells PLoS ONE 2013-04-05 [PMID: 23637756] (Bioassay, Human, Mouse, Rat)
• Dessarthe B, Thedrez A, Latouche J, Cabillic F, Drouet A, Daniel P, de La Pintiere C, Catros V, Toutirais O CRTAM receptor engagement by Necl-2 on tumor cells triggers cell death of activated Vgamma9Vdelta2 T cells. J Immunol, 2013-03-25;190(9):4868-76. 2013-03-25 [PMID: 23530148] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CRTAM
• Uniprot:
  • O95727()