Recombinant Human CRTAC1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CRTAC1 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.25-1.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human CRTAC1 protein
  Ser28-Cys661, with a C-terminal 6-His tag
• Accession #: Q9NQ79
• N-terminal Sequence: Ser28
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CRTAC1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 83-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CRTAC1 Protein, CF

• ASPIC
• ASPIC1
• ASPIC1CEP-68acidic secreted protein in cartilage
• cartilage acidic protein 1
• CEP68
• chondrocyte expressed protein 68 kDa CEP-68,68 kDa chondrocyte-expressed protein
• CRTAC1
• FLJ10320

Background

Cartilage acidic protein 1 (CRTAC1), also known as CEP‑68, is a 95 kDa glycosylated protein that contains four FG‑GAP domains and one EGF‑like domain. An integrin‑binding RGD motif is present in the human protein but absent in the mouse and rat proteins. Mature human CRTAC1 shares 89% amino acid sequence identity with mouse and rat CRTAC. It is secreted by chondrocytes into the extracellular matrix of cartilage (1, 2). It is also up‑regulated in type II alveolar epithelial cells upon dexamethasone exposure (3). CRTAC1B, otherwise known as LOTUS, is an 85 kDa alternatively spliced isoform that has a substituted C‑terminal region with a potential transmembrane segment (2, 4). CRTAC1B is preferentially expressed in select regions of the brain on neuronal growth cones and neurite shafts (2, 4). It associates with the Nogo Receptor (NgR) and prevents Nogo‑induced growth cone collapse (4, 5). In mouse, CRTAC1B plays an important role in axonal bundling and formation of the lateral olfactory tract (4).

1. Steck, E. et al. (2001) Biochem. J. 353:169.
2. Steck, E. et al. (2007) Matrix Biol. 26:30.
3. Ballard, P.L. et al. (2010) Am. J. Physiol. Lung Cell. Mol. Physiol. 299:L36.
4. Sato, Y. et al. (2011) Science 333:769.
5. Kurihara, Y. et al. (2012) Biochem. Biophys. Res. Commun. 418:390.

Publications for CRTAC1 (5234-CR)(1)

Publication using 5234-CR

• Johansson, MW;Balnis, J;Muehlbauer, LK;Bukhman, YV;Stefely, MS;Overmyer, KA;Vancavage, R;Tiwari, A;Adhikari, AR;Feustel, PJ;Schwartz, BS;Coon, JJ;Stewart, R;Jaitovich, A;Mosher, DF; Decreased plasma cartilage acidic protein 1 in COVID-19 Physiological reports 2023-09-01 [PMID: 37667413] (ELISA Standard, Human)

Bioinformatics

• Gene Symbol: CRTAC1
• Uniprot:
  • Q9NQ79()