Recombinant Human Contactin-4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Contactin-4 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 400 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human Contactin-4 protein
  Asp19-Gly1001, with a C-terminal 10-His tag
• Accession #: Q8IWV2
• N-terminal Sequence: Asp19
• Protein/Peptide Type: Recombinant Proteins
• Gene: CNTN4
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 110.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 145-155 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Contactin-4 Protein, CF

• AXCAM
• axonal-associated cell adhesion molecule
• BIG-2
• BIG-2Brain-derived immunoglobulin superfamily protein 2
• CNTN4
• contactin 4
• Contactin4
• Contactin-4
• MGC33615
• neural cell adhesion protein BIG-2

Background

Contactin-4 (CNTN4), also known as BIG-2 (Brain-derived Immunoglobulin Superfamily molecule 2), is an axonal cell adhesion molecule that belongs to the contactin family, a subfamily of the Ig superfamily (1 - 3). The contactin family comprises six members (CNTN1/F3, CNTN2/TAG-1, CNTN3/BIG-1, CNTN4/BIG-2, CNTN5/NB-2 and CNTN6/NB-3) and are characterized by the presence of six Ig-like domains, four fibronectin type III-like repeats, and a glycosylphosphatidylinositol (GPI)-anchoring domain. Contactin family proteins exist as membrane-bound proteins, but can also be released as soluble proteins by GPI-specific phospholipase D. Soluble Contactins are functionsl proteins that are able to promote neurite outgrowth. Human CNTN4 has been mapped to chromosome 3p26 - p25. Three alternative transcripts of CNTN4, encoding isoforms a, b, and c precursor proteins containing 1026, 282, and 698 amino acid (aa) residues, respectively, have been described. Human CNTN4 isoform a shares 94% aa sequence identity with its rat homolog. It also shares from 44 - 66% aa sequence identity with other CNTN family members. CNTN family members display overlapping but distinct expression patterns. CNTN4 expression is detected in multiple organs including brains, pancreas, kidney, aorta, small intestine, thyroid, uterus and testis. However, expression of the 282 aa isoform b is primarily restricted to the brain. CNTN4 has been suggested to play important roles in the formation of neuronal networks during nervous sytems development. Disruption of CNTN4 has been implicated in the 3p deletion syndrome characterized by growth failure, developmental delay, and mental retardation. CNTN-4 expression is induced in human neuroblastoma tumor cells treated with retinoic acid and may be responsible for the the neuritogenic response of tumor cells to retinoids. R&D Systems' recombinant CNTN4 shows in vitro neurite outgrowth promoting activity when used as an immobilized substrate for hippocampal neuron outgrowth.

1. Fernandez, T. et al. (2004) Am. J. Genet. 74:1286.
2. Hasnford, L.M. et al. (2003) Cytogenet. Genome Res. 101:17.
3. Yoshihara, Y. et al. (1995) J. Neurobiology 28:51.

Publications for Contactin-4 (2205-CN)(1)

Publication using 2205-CN

• K Mikulska-R, AJ Kulik, C Benadiba, I Bahar, G Dietler, W Nowak Nanomechanics of multidomain neuronal cell adhesion protein contactin revealed by single molecule AFM and SMD Sci Rep, 2017-08-18;7(1):8852. 2017-08-18 [PMID: 28821864]

Bioinformatics

• Gene Symbol: CNTN4
• Uniprot:
  • Q8IWV2()