Recombinant Human Complement Component C5a Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Complement Component C5a Protein Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Klos, A. et al. (1992) Biochemistry 31:11274. The ED50 for this effect is 1.5-5 ng/mL.
Source
E. coli-derived human Complement Component C5a protein
Thr678-Arg751
Accession #
N-terminal Sequence
Thr678
Protein/Peptide Type
Recombinant Proteins
Gene
C5
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2037-C5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Component C5a Protein

  • C5
  • C5A
  • Complement Component C5a
  • HE

Background

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

  1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

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Publications for Complement Component C5a (2037-C5)(13)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, Hemofiltration Procedure, Protein Filtration, Surface Plasmon Resonance.


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Binding Assay
(1)
Bioassay
(9)
Hemofiltration Procedure
(1)
Protein Filtration
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 2037-C5 Applications Species
Y Zhang, Y Cao, L Zhang, C Feng, G Zhou, G Wen Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR Biosci. Rep., 2018;0(0):. 2018 [PMID: 29685955] (Bioassay, Human) Bioassay Human
MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018;13(1):e0191676. 2018 [PMID: 29370247] (Protein Filtration, Human) Protein Filtration Human
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human) Hemofiltration Procedure Human
EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells Oncotarget, 2017;8(1):1438-1448. 2017 [PMID: 27903965] (Bioassay, Human) Bioassay Human
Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth Sci Rep, 2016;6(0):31799. 2016 [PMID: 27539194] (Bioassay, Human) Bioassay Human
Yatime, Laure, Maasch, Christia, Hoehlig, Kai, Klussmann, Sven, Andersen, Gregers, Vater, Axel Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun, 2015;6(0):6481. 2015 [PMID: 25901944] (Bioassay, Mouse) Bioassay Mouse
Chmilewsky F, Jeanneau C, Laurent P, About I Pulp fibroblasts synthesize functional complement proteins involved in initiating dentin-pulp regeneration. Am J Pathol, 2014;184(7):1991-2000. 2014 [PMID: 24814102] (Bioassay, Human) Bioassay Human
Jensen B, Frandsen P, Raaby E, Schiotz P, Skov P, Poulsen L Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells. J Leukoc Biol, 2014;95(6):893-901. 2014 [PMID: 24493825] (Bioassay, Human) Bioassay Human
Hoehlig, Kai, Maasch, Christia, Shushakova, Nelli, Buchner, Klaus, Huber-Lang, Markus, Purschke, Werner G, Vater, Axel, Klussmann, Sven A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther, 2013;21(12):2236-46. 2013 [PMID: 23887360] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration. J Transl Med, 2011;9(0):111. 2011 [PMID: 21762495] (Bioassay, Human) Bioassay Human
Show All 13 Publications.

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Bioinformatics

Gene Symbol C5
Entrez
Uniprot