Recombinant Human Complement Component C5a Protein


There are currently no images for Complement Component C5a (2037-C5).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Complement Component C5a Protein Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 1.5-5 ng/mL.
E. coli-derived human Complement Component C5a protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
8.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
2037-C5 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Component C5a Protein

  • C5
  • C5A
  • Complement Component C5a
  • HE


Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

  1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, RM
Applications: WB, Flow, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC
Species: Hu
Applications: WB, Simple Western
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: Bioactivity

Publications for Complement Component C5a (2037-C5)(14)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 6 applications: Binding Assay, Bioassay, Cell Culture, Hemofiltration Procedure, Protein Filtration, Surface Plasmon Resonance.

Filter By Application
Binding Assay
Cell Culture
Hemofiltration Procedure
Protein Filtration
Surface Plasmon Resonance
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 2037-C5 Applications Species
J Paupert, E Espinosa, N Cenac, V Robert, P Laharrague, SM Evrard, L Casteilla, A Lorsignol, B Cousin Rapid and Efficient Production of Human Functional Mast Cells Through a Three-Dimensional Culture of Adipose Tissue-Derived Stromal Vascular Cells J. Immunol., 2018;0(0):. 2018 [PMID: 30446570] (Cell Culture, Human) Cell Culture Human
Y Zhang, Y Cao, L Zhang, C Feng, G Zhou, G Wen Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR Biosci. Rep., 2018;0(0):. 2018 [PMID: 29685955] (Bioassay, Human) Bioassay Human
MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018;13(1):e0191676. 2018 [PMID: 29370247] (Protein Filtration, Human) Protein Filtration Human
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human) Hemofiltration Procedure Human
EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells Oncotarget, 2017;8(1):1438-1448. 2017 [PMID: 27903965] (Bioassay, Human) Bioassay Human
Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth Sci Rep, 2016;6(0):31799. 2016 [PMID: 27539194] (Bioassay, Human) Bioassay Human
Yatime, Laure, Maasch, Christia, Hoehlig, Kai, Klussmann, Sven, Andersen, Gregers, Vater, Axel Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun, 2015;6(0):6481. 2015 [PMID: 25901944] (Bioassay, Mouse) Bioassay Mouse
Chmilewsky F, Jeanneau C, Laurent P, About I Pulp fibroblasts synthesize functional complement proteins involved in initiating dentin-pulp regeneration. Am J Pathol, 2014;184(7):1991-2000. 2014 [PMID: 24814102] (Bioassay, Human) Bioassay Human
Jensen B, Frandsen P, Raaby E, Schiotz P, Skov P, Poulsen L Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells. J Leukoc Biol, 2014;95(6):893-901. 2014 [PMID: 24493825] (Bioassay, Human) Bioassay Human
Hoehlig, Kai, Maasch, Christia, Shushakova, Nelli, Buchner, Klaus, Huber-Lang, Markus, Purschke, Werner G, Vater, Axel, Klussmann, Sven A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther, 2013;21(12):2236-46. 2013 [PMID: 23887360] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Show All 14 Publications.

Reviews for Complement Component C5a (2037-C5) (0)

There are no reviews for Complement Component C5a (2037-C5). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Complement Component C5a (2037-C5) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Complement Component C5a Products

Bioinformatics Tool for Complement Component C5a (2037-C5)

Discover related pathways, diseases and genes to Complement Component C5a (2037-C5). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Complement Component C5a (2037-C5)

Discover more about diseases related to Complement Component C5a (2037-C5).

Pathways for Complement Component C5a (2037-C5)

View related products by pathway.

PTMs for Complement Component C5a (2037-C5)

Learn more about PTMs related to Complement Component C5a (2037-C5).

Blogs on Complement Component C5a

There are no specific blogs for Complement Component C5a, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human Complement Component C5a Protein and receive a gift card or discount.


Gene Symbol C5