Recombinant Human Complement Component C5a Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Complement Component C5a Protein, CF Summary

• Details of Functionality: Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Klos, A. et al. (1992) Biochemistry 31:11274. The ED₅₀ for this effect is 1.5-5 ng/mL.
• Source: E. coli-derived human Complement Component C5a protein
  Thr678-Arg751
• Accession #: P01031
• N-terminal Sequence: Thr678
• Protein/Peptide Type: Recombinant Proteins
• Gene: C5
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 8.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Component C5a Protein, CF

• C5
• C5A
• Complement Component C5a
• HE

Background

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

Publications for Complement Component C5a (2037-C5/CF)(13)

Publications using 2037-C5/CF

• Y Zhang, Y Cao, L Zhang, C Feng, G Zhou, G Wen Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR Biosci. Rep., 2018;0(0):. 2018 [PMID: 29685955] (Bioassay, Human)
• MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018;13(1):e0191676. 2018 [PMID: 29370247] (Protein Filtration, Human)
• Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human)
• EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells Oncotarget, 2017;8(1):1438-1448. 2017 [PMID: 27903965] (Bioassay, Human)
• Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth Sci Rep, 2016;6(0):31799. 2016 [PMID: 27539194] (Bioassay, Human)
• Yatime, Laure, Maasch, Christia, Hoehlig, Kai, Klussmann, Sven, Andersen, Gregers, Vater, Axel Structural basis for the targeting of complement anaphylatoxin C5a using a mixed L-RNA/L-DNA aptamer. Nat Commun, 2015;6(0):6481. 2015 [PMID: 25901944] (Bioassay, Mouse)
• Chmilewsky F, Jeanneau C, Laurent P, About I Pulp fibroblasts synthesize functional complement proteins involved in initiating dentin-pulp regeneration. Am J Pathol, 2014;184(7):1991-2000. 2014 [PMID: 24814102] (Bioassay, Human)
• Jensen B, Frandsen P, Raaby E, Schiotz P, Skov P, Poulsen L Molecular and stimulus-response profiles illustrate heterogeneity between peripheral and cord blood-derived human mast cells. J Leukoc Biol, 2014;95(6):893-901. 2014 [PMID: 24493825] (Bioassay, Human)
• Hoehlig, Kai, Maasch, Christia, Shushakova, Nelli, Buchner, Klaus, Huber-Lang, Markus, Purschke, Werner G, Vater, Axel, Klussmann, Sven A novel C5a-neutralizing mirror-image (l-)aptamer prevents organ failure and improves survival in experimental sepsis. Mol Ther, 2013;21(12):2236-46. 2013 [PMID: 23887360] (Surface Plasmon Resonance, N/A)
• Liu B, Wei L, Meyerle C, Tuo J, Sen HN, Li Z, Chakrabarty S, Agron E, Chan CC, Klein ML, Chew E, Ferris F, Nussenblatt RB Complement component C5a Promotes Expression of IL-22 and IL-17 from Human T cells and its Implication in Age-related Macular Degeneration. J Transl Med, 2011;9(0):111. 2011 [PMID: 21762495] (Bioassay, Human)
• Ruan BH, Li X, Winkler AR, Cunningham KM, Kuai J, Greco RM, Nocka KH, Fitz LJ, Wright JF, Pittman DD, Tan XY, Paulsen JE, Lin LL, Winkler DG Complement C3a, CpG oligos, and DNA/C3a complex stimulate IFN-alpha production in a receptor for advanced glycation end product-dependent manner. J. Immunol., 2010;185(7):4213-22. 2010 [PMID: 20817881] (Binding Assay, Human)
• Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood, 2008;112(13):5026-36. 2008 [PMID: 18799722] (Bioassay, Human)
• Rittirsch D, Flierl MA, Nadeau BA, Day DE, Huber-Lang M, Mackay CR, Zetoune FS, Gerard NP, Cianflone K, Kohl J, Gerard C, Sarma JV, Ward PA Functional roles for C5a receptors in sepsis. Nat. Med., 2008;14(5):551-7. 2008 [PMID: 18454156] (Bioassay, Human)

Bioinformatics

• Gene Symbol: C5
• Entrez:
  • 727(Human)
  • 15139(Mouse)
• Uniprot:
  • P01031()