Recombinant Human Complement Component C5a Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Complement Component C5a Protein, CF Summary

Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 1.5-5 ng/mL.
Source
E. coli-derived human Complement Component C5a protein
Thr678-Arg751
Accession #
N-terminal Sequence
Thr678
Protein/Peptide Type
Recombinant Proteins
Gene
C5
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
8.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2037-C5/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Complement Component C5a Protein, CF

  • C5
  • C5A
  • Complement Component C5a
  • HE

Background

Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.

  1. Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
  2. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
  3. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
  4. Okinaga, S. et al. (2003) Biochemistry 42:9406.
  5. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

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2037-C5/CF
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Applications: Bioactivity

Publications for Complement Component C5a (2037-C5/CF)(20)

We have publications tested in 4 confirmed species: Human, Mouse, Mosquito, N/A.

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, Chemoattractant, Hemofiltration Procedure, Protein Filtration, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(1)
Bioassay
(14)
Cell Culture
(1)
Chemoattractant
(1)
Hemofiltration Procedure
(1)
Protein Filtration
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(17)
Mouse
(1)
Mosquito
(1)
N/A
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 2037-C5/CF Applications Species
J Liu, X Wei, J Hu, X Tan, X Kang, L Gao, N Li, X Shi, M Yuan, W Hu, M Liu Different concentrations of C5a affect human dental pulp mesenchymal stem cells differentiation BMC Oral Health, 2021;21(1):470. 2021 [PMID: 34560867] (Bioassay, Human) Bioassay Human
D Medica, R Franzin, A Stasi, G Castellano, M Migliori, V Panichi, F Figliolini, L Gesualdo, G Camussi, V Cantaluppi Extracellular Vesicles Derived from Endothelial Progenitor Cells Protect Human Glomerular Endothelial Cells and Podocytes from Complement- and Cytokine-Mediated Injury Cells, 2021;10(7):. 2021 [PMID: 34359843] (Bioassay, Human) Bioassay Human
S Silawal, B Kohl, J Shi, G Schulze-Ta Complement Regulation in Human Tenocytes under the Influence of Anaphylatoxin C5a International Journal of Molecular Sciences, 2021;22(6):. 2021 [PMID: 33803624] (Bioassay, Human) Bioassay Human
N Kashiwagi, F Saito, H Maegawa, K Kaneda Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response - In vitro and in vivo results Cytokine, 2021;139(0):155410. 2021 [PMID: 33395614] (Chemoattractant, Human) Chemoattractant Human
GI Giraldo-Ca, A Calle-Tobó, P Rozo-López, TM Colpitts, Y Park, GL Rua-Uribe, B Londono-Re Transcriptome of the Aedes aegypti Mosquito in Response to Human Complement Proteins Int J Mol Sci, 2020;21(18):. 2020 [PMID: 32916828] (Bioassay, Mosquito) Bioassay Mosquito
S Ohlsson, L Holm, C Hansson, SM Ohlsson, L Gunnarsson, Å Pettersson, L Skattum Neutrophils from ANCA-associated vasculitis patients show an increased capacity to activate the complement system via the alternative pathway after ANCA stimulation PLoS ONE, 2019;14(6):e0218272. 2019 [PMID: 31216309] (Bioassay, Human) Bioassay Human
J Paupert, E Espinosa, N Cenac, V Robert, P Laharrague, SM Evrard, L Casteilla, A Lorsignol, B Cousin Rapid and Efficient Production of Human Functional Mast Cells Through a Three-Dimensional Culture of Adipose Tissue-Derived Stromal Vascular Cells J. Immunol., 2018;0(0):. 2018 [PMID: 30446570] (Cell Culture, Human) Cell Culture Human
Y Zhang, Y Cao, L Zhang, C Feng, G Zhou, G Wen Apigenin inhibits C5a-induced proliferation of human nasopharyngeal carcinoma cells through down-regulation of C5aR Biosci. Rep., 2018;0(0):. 2018 [PMID: 29685955] (Bioassay, Human) Bioassay Human
MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads PLoS ONE, 2018;13(1):e0191676. 2018 [PMID: 29370247] (Protein Filtration, Human) Protein Filtration Human
Benjamin Malard Adsorption as a Contributor for Inflammatory Mediators Removal by Different Hemofiltration Membranes: A Pilot Study Artif Organs, 2016;0(0):. 2016 [PMID: 27911005] (Hemofiltration Procedure, Human) Hemofiltration Procedure Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol C5
Uniprot