Recombinant Human Complement Component C5a Protein, CF Summary
Details of Functionality
Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. Gerard, NP and Gerard, C. (1990) Biochemistry 29:9274. The ED50 for this effect is 1.5-5 ng/mL.
Source
E. coli-derived human Complement Component C5a protein Thr678-Arg751
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
8.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using 2037-C5/CF in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Complement Component C5a Protein, CF
C5
C5A
Complement Component C5a
HE
Background
Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C‑terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Gerard, C. and N.P. Gerard, (1994) Annu. Rev. Immunol. 12:775.
DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629.
Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115.
Okinaga, S. et al. (2003) Biochemistry 42:9406.
Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.
Publications for Complement Component C5a (2037-C5/CF)(20)
We have publications tested in 4 confirmed species: Human, Mouse, Mosquito, N/A.
We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, Chemoattractant, Hemofiltration Procedure, Protein Filtration, Surface Plasmon Resonance.
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