Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Additional Information | Biotinylated IL-2 R gamma Fc Chimera Avi-tag |
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Details of Functionality | Measured by its binding ability in a functional ELISA. When Recombinant Human CD25/IL-2R alpha Protein
(Catalog #
223-2A)
and Recombinant Human IL-2R beta Protein
(Catalog #
223-2B)
are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi‑tag (Catalog # AVI11045) that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL‑2 Protein
(Catalog #
202-IL). |
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Source | Chinese Hamster Ovary cell line, CHO-derived human Common gamma Chain/IL-2 R gamma protein
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Accession # | |||||||||
N-terminal Sequence | Leu23 |
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Structure / Form | Disulfide-linked
homodimer Biotinylated via Avi-tag |
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Protein/Peptide Type | Recombinant Proteins |
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Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
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Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 56 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE | 85-95 kDa, under reducing conditions. |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 500 μg/mL in PBS. |
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene. Our Avi-tag Biotinylated IL-2 R gamma features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
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Uniprot |
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